Phylogenetic analysis and full-length characterization of S1 gene of IS-1494 (Variant 2) like infectious bronchitis virus isolates, Iran, 2015

Virology reports Pub Date : 2016-12-01 DOI:10.1016/j.virep.2016.05.002

Hamzeh Nabavi , Vahid Karimi , Arash Ghalyanchilangeroudi , Saeed Shateri , Waleed Seger , Hamideh Najafi

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引用次数: 5

Abstract

Infectious bronchitis virus (IBV), a major pathogen of commercial poultry flocks, circulates in the form of different genotypes. Three IB viruses were isolated from broiler chickens showing respiratory and renal lesions. The isolates were characterized by reverse transcriptase polymerase chain reaction and sequence analysis of the Full-length of the S1. Three isolates were belonged to Variant 2 like (IS/1494 like) IBV genotype. Phylogenetic analysis showed that Variant 2 like isolates formed two clusters and the Iranian and Iraqi isolates were included in the cluster II. Cluster I composed of Israeli, Egyptian and Turkish Variant 2 like IBV Isolates. Three hyper variable regions (HVR) of S1 were determined. The Most variation was seen in HVR2. The findings emphasize the importance of continuous monitoring of IBV, in addition to adjust diagnostic methods, molecular epidemiological studies, development and use of vaccines which are adapted to the changing disease scenario.

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传染性支气管炎病毒IS-1494(变异2)样分离株S1基因的系统发育分析及全长鉴定，伊朗，2015

传染性支气管炎病毒(IBV)是商品禽群的主要病原体，以不同基因型的形式传播。从出现呼吸道和肾脏病变的肉鸡中分离出3种IB病毒。通过逆转录酶聚合酶链反应和S1全长序列分析对分离物进行了鉴定。3株IBV为变异2样(IS/1494样)型。系统发育分析表明，2型变异样分离株形成2个聚类，伊朗和伊拉克分离株属于2型聚类。群集1由以色列、埃及和土耳其变体2组成，类似IBV分离株。测定了S1的三个高变区(HVR)。变异最大的是HVR2。研究结果强调，除了调整诊断方法、分子流行病学研究、开发和使用适应不断变化的疾病情况的疫苗外，还必须持续监测IBV。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Virology reports

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