Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2012-10-01 DOI:10.1016/j.inmuno.2012.09.003

María C. Grosso , Romina V. Bellingeri , Rüdiger Schade , Adriana B. Vivas

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引用次数: 2

Abstract

The aim of this study was to evaluate the effects of passive immunization with polyclonal anti-swine early pregnancy factor (EPF) antibody in pregnant rats during the pre-implantation period, and to analyse the regional distribution of EPF in placenta of rats. The immunodetection of EPF in rat placenta, as well as fertilization, development and immunological parameters, was evaluated. The average number of embryos and the embryo/corpora lutea ratio of rats treated with anti-EPF antibody were significantly lower than control groups. Also, as expected, the embryos showed a delay in their development. Furthermore, while IL-10 and INF-γ levels increased, serum and placental TGF-β decreased significantly in the group treated with anti-EPF. EPF was present in giant and decidual cells, as well as in blood vessels and trophoblastic lacuna cells, as demonstrated by positive immunostaining. This study provides new results on the function and location of EPF, and has demonstrated the usefulness of polyclonal anti-swine EPF antibody.

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通过被动免疫来中和早孕因子会改变胚胎的正常发育和细胞因子的平衡

本研究旨在评价多克隆抗猪早孕因子(EPF)抗体被动免疫对着床前妊娠大鼠的影响，并分析EPF在大鼠胎盘中的区域分布。对EPF在大鼠胎盘中的免疫检测及受精、发育和免疫学指标进行了评价。抗epf抗体处理大鼠的平均胚胎数和胚胎/黄体比显著低于对照组。而且，正如预期的那样，胚胎的发育出现了延迟。此外，抗epf组在IL-10和INF-γ水平升高的同时，血清和胎盘TGF-β水平显著降低。免疫染色阳性显示EPF存在于巨细胞和蜕细胞，以及血管和滋养层陷窝细胞。本研究为猪EPF的功能和定位提供了新的结果，并证明了多克隆抗猪EPF抗体的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Inmunologia (Barcelona, Spain : 1987)

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