The membrane complexome of a new Pseudomonas strain during growth on lysogeny broth medium and medium containing glucose or phenol

Q4 Biochemistry, Genetics and Molecular Biology
Antigoni Nikolaki , Anastasia Papadioti , Katerina Arvaniti , Eleni Kassotaki , Julian D. Langer , Georgios Tsiotis
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引用次数: 3

Abstract

In this study, we have performed a systematic analysis of Pseudomonas sp. strain phDV1 membrane protein complexes by growing the strain in lysogeny broth medium, and medium containing glucose or phenol as sole carbon sources. In order to study the membrane complexome, we developed an approach for the extraction and the analysis of the membrane protein complexes in native conditions. Our strategy involves (a) enrichment of the membrane proteome from Pseudomonas sp. strain phDV1 by two washing steps; (b) solubilization using n-dodecyl-β-maltoside; (c) a combination of BN-PAGE with Tricine-SDS-PAGE; and (d) protein identification of tryptic peptides by mass spectrometry.

Abstract Image

一株新假单胞菌在溶菌肉汤培养基和含葡萄糖或苯酚培养基上生长过程中的膜复合物
在本研究中,我们对假单胞菌菌株phDV1膜蛋白复合物进行了系统的分析,通过在溶原肉汤培养基和含葡萄糖或苯酚作为唯一碳源的培养基中培养菌株。为了研究膜复合物,我们开发了一种在天然条件下提取和分析膜蛋白复合物的方法。我们的策略包括:(a)通过两个洗涤步骤富集假单胞菌菌株phDV1的膜蛋白质组;(b) n-十二烷基-β-麦芽糖苷增溶;(c) BN-PAGE与Tricine-SDS-PAGE的组合;(d)质谱法鉴定色氨酸蛋白。
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来源期刊
EuPA Open Proteomics
EuPA Open Proteomics Biochemistry, Genetics and Molecular Biology-Biochemistry
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