Evaluación de la genotoxicidad inducida por la administración repetida de anestésicos locales: un estudio experimental en ratones

Brazilian Journal of Anesthesiology (Edicion en Espanol) Pub Date : 2015-01-01 DOI:10.1016/j.bjanes.2013.07.008

Gisele Alborghetti Nai , Mariliza Casanova de Oliveira , Graziela de Oliveira Tavares , Laís Fabrício Fonseca Pereira , Nádia Derli Salvador Lemes Soares , Patrícia Gatti Silva

{"title":"Evaluación de la genotoxicidad inducida por la administración repetida de anestésicos locales: un estudio experimental en ratones","authors":"Gisele Alborghetti Nai , Mariliza Casanova de Oliveira , Graziela de Oliveira Tavares , Laís Fabrício Fonseca Pereira , Nádia Derli Salvador Lemes Soares , Patrícia Gatti Silva","doi":"10.1016/j.bjanes.2013.07.008","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and objective</h3><p>Previous studies regarding the effects of some local anesthetics have suggested these agents may cause genetic damage. However, they have not been tested for genotoxicity related to repetitive administration. The aim of this study was to evaluate the genotoxic potential of local anesthetics upon repetitive administration.</p></div><div><h3>Methods</h3><p>80 male Wistar rats were allocated into: group A - 16 rats injected intraperitoneally (IP) with lidocaine hydrochloride 2%; group B - 16 rats IP injected with mepivacaine 2%; group C - 16 rats IP injected with articaine 4%; group D - 16 rats IP injected with prilocaine 3% (6.0mg.kg<sup>–1</sup>); group E - 8 rats subcutaneously injected with a single dose of cyclophosphamide; and group F - 8 rats IP injected with saline. Eight rats from groups A to D received a single dose of anaesthetic on day 1 of the experiment; the remaining rats were injected once a day for 5 days.</p></div><div><h3>Results</h3><p>The median number of micronuclei in the local anesthetics groups exposed for one or 5 days ranged from 0.00 to 1.00, in the cyclophosphamide-exposed group was 10.00, and the negative control group for 1 and 5 days was 1.00 and 0.00, respectively (<em>p</em>  =0.0001), but not between the negative control group and the local anesthetic groups (<em>p</em>  <!-->0.05).</p></div><div><h3>Conclusion</h3><p>No genotoxicity effect was observed upon repetitive exposure to any of the local anesthetics evaluated.</p></div>","PeriodicalId":100199,"journal":{"name":"Brazilian Journal of Anesthesiology (Edicion en Espanol)","volume":"65 1","pages":"Pages 21-26"},"PeriodicalIF":0.0000,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjanes.2013.07.008","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Anesthesiology (Edicion en Espanol)","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2255496313001803","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

Abstract

Background and objective

Previous studies regarding the effects of some local anesthetics have suggested these agents may cause genetic damage. However, they have not been tested for genotoxicity related to repetitive administration. The aim of this study was to evaluate the genotoxic potential of local anesthetics upon repetitive administration.

Methods

80 male Wistar rats were allocated into: group A - 16 rats injected intraperitoneally (IP) with lidocaine hydrochloride 2%; group B - 16 rats IP injected with mepivacaine 2%; group C - 16 rats IP injected with articaine 4%; group D - 16 rats IP injected with prilocaine 3% (6.0 mg.kg^–1); group E - 8 rats subcutaneously injected with a single dose of cyclophosphamide; and group F - 8 rats IP injected with saline. Eight rats from groups A to D received a single dose of anaesthetic on day 1 of the experiment; the remaining rats were injected once a day for 5 days.

Results

The median number of micronuclei in the local anesthetics groups exposed for one or 5 days ranged from 0.00 to 1.00, in the cyclophosphamide-exposed group was 10.00, and the negative control group for 1 and 5 days was 1.00 and 0.00, respectively (p < 0.0001). A significant difference in the number of micronuclei was observed between the cyclophosphamide group and all local anesthetic groups (p = 0.0001), but not between the negative control group and the local anesthetic groups (p > 0.05).

Conclusion

No genotoxicity effect was observed upon repetitive exposure to any of the local anesthetics evaluated.

查看原文本刊更多论文

重复给药局麻药致遗传毒性的评价:小鼠实验研究

背景与目的以往关于局部麻醉药作用的研究表明，这些药物可能导致遗传损伤。然而，它们还没有被测试与重复给药有关的遗传毒性。本研究的目的是评估反复使用局麻药的遗传毒性潜力。方法80只雄性Wistar大鼠分为:A ~ 16只大鼠腹腔注射盐酸利多卡因2%;B - 16组大鼠IP注射2%甲哌卡因;C - 16组大鼠IP注射阿替卡因4%;D - 16组大鼠IP注射丙胺卡因3% (6.0 mg.kg-1);E - 8组大鼠皮下注射单剂量环磷酰胺;F - 8组大鼠IP注射生理盐水。A ~ D组8只大鼠在实验第1天给予单剂量麻醉;其余大鼠每天注射1次，连续5天。结果局麻药暴露1 d和5 d组微核数中位数为0.00 ~ 1.00，环磷酰胺暴露组为10.00，阴性对照组1 d和5 d微核数中位数分别为1.00和0.00 (p <0.0001)。环磷酰胺组与各局麻药组间微核数目差异有统计学意义(p = 0.0001)，阴性对照组与局麻药组间无统计学意义(p >0.05)。结论所有局麻药重复暴露均无遗传毒性作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Brazilian Journal of Anesthesiology (Edicion en Espanol)

自引率

0.00%

发文量