A missense mutation affects the mobility of high molecular weight glutenin Dy10 subunit in SDS-PAGE

Q1 Agricultural and Biological Sciences

Agri Gene Pub Date : 2016-12-01 DOI:10.1016/j.aggene.2016.09.001

Yan Wang , Qing-Yun Zheng , Zhen-Ru Guo , Yuan-Yuan Qiao , Yong-Li Cao , Cai-Hong Liu , Bin-Jie Xu , Zheng-Yuan Wei , Lu-Juan Zong , Chen Chen , Ya-Nan Han , Qing Chen , Xi Gong , Shi-Yu Hua , You-Liang Zheng , Peng-Fei Qi , Yu-Ming Wei , Xiu-Jin Lan , Ji-Rui Wang

引用次数: 6

Abstract

High-molecular-weight glutenin subunits (HMW-GS) are important in wheat end-use quality. We characterized a HMW-GS Dy10 mutant subunit with faster mobility than Dy10 in SDS-PAGE, and with equal mobility to Dy10 in urea-containing SDS-PAGE gel. A missense mutation was identified in the mutant subunit, which led to substitution of the 328th amino acid residue from ser (S) to phe (F) in the repetitive domain. The Dy10 subunit containing the missense mutation was designated as Dy10-m328SF (Genbank No. KU934284). Our results indicate that a single amino acid substitution in the repetitive domain can change the mobility of Dy10.

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错义突变影响SDS-PAGE中高分子量谷蛋白Dy10亚基的迁移

高分子量谷蛋白亚基(HMW-GS)在小麦最终使用品质中起着重要作用。我们鉴定了一个HMW-GS Dy10突变亚基，它在SDS-PAGE中迁移速度比Dy10快，在含尿素的SDS-PAGE凝胶中与Dy10具有相同的迁移速度。在突变亚基中发现了一个错义突变，导致重复结构域的第328个氨基酸残基从ser (S)替换为phe (F)。含有错义突变的Dy10亚基被命名为Dy10- m328sf (Genbank No. 328sf)。KU934284)。我们的研究结果表明，重复结构域的单个氨基酸替换可以改变Dy10的迁移率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.