Association of TLR4 gene variants with milk production traits in crossbred cattle

Q1 Agricultural and Biological Sciences

Agri Gene Pub Date : 2016-08-01 DOI:10.1016/j.aggene.2016.07.002

Chinmoy Mishra , Subodh Kumar , H.M. Yathish , Rajni Choudhary , Amit Kumar

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引用次数: 1

Abstract

The identification of dairy cows with higher milk yield potential has been pursued for many years in different herds. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within major genes may affect milk production and consequently result economic benefit to the farmers. Toll-like receptor 4 (TLR4) is an immunogenic gene activating innate and adaptive immune responses which is present in very close proximity to the quantitative trait loci (QTL) affecting milk production traits. The present research work was designed to explore the possible genetic variations in 5′ upstream region of TLR4 gene in Vrindavani cattle and their possible associations with milk production traits viz. total lactation milk yield, 305 days milk yield and lactation length. Nucleotide variability in 5′ upstream region of TLR4 gene was identified by PCR-RFLP and subsequent sequencing. The haplotypes were constructed using those SNPs. The association of these haplotypes with production traits was studied using SAS 9.3 software. Statistically significant association between these haplotypes and TLMY was observed. However, no significant association was found between haplotypes with MY305 and LL.

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杂交牛TLR4基因变异与产奶量性状的关系

多年来，人们一直在不同的奶牛群中寻找具有较高产奶量潜力的奶牛。基因组学研究表明，主要基因内的单核苷酸多态性(snp)可能影响牛奶产量，从而给农民带来经济效益。toll样受体4 (TLR4)是一种激活先天性和适应性免疫应答的免疫原性基因，与影响产奶量性状的数量性状位点(QTL)非常接近。本研究旨在探讨Vrindavani牛TLR4基因上游5′区可能存在的遗传变异及其与泌乳总产奶量、305日产奶量和泌乳时长等产乳性状的关系。采用PCR-RFLP技术鉴定TLR4基因上游5′区核苷酸变异，并进行测序。利用这些snp构建单倍型。利用SAS 9.3软件研究这些单倍型与生产性状的关联。这些单倍型与TLMY之间存在统计学上显著的相关性。然而，单倍型与MY305和LL之间没有发现显著的关联。

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Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.