Changes in transferrin gene expression after exposure to iron and Aeromonas hydrophila infection in yellow snapper (Lutjanus argentiventris)

Q1 Agricultural and Biological Sciences

Agri Gene Pub Date : 2016-08-01 DOI:10.1016/j.aggene.2016.04.002

Martha Reyes-Becerril, Carlos Angulo, Miriam Angulo, Felipe Ascencio-Valle

{"title":"Changes in transferrin gene expression after exposure to iron and Aeromonas hydrophila infection in yellow snapper (Lutjanus argentiventris)","authors":"Martha Reyes-Becerril, Carlos Angulo, Miriam Angulo, Felipe Ascencio-Valle","doi":"10.1016/j.aggene.2016.04.002","DOIUrl":null,"url":null,"abstract":"<div>Transferrin (Tfa) plays an important role in iron regulation and is also known to be involved in response to infections. In this study, the full-length cDNA of the transferrin gene from Lutjanus argentiventris was cloned and characterized. The full-length cDNA of the tfa was 2482h in juveniles exposed to iron (Fe2x) during Aeromonas hydrophila infection. In general, the expression of tfa decreased in liver and intestine and increased in gill and skin in the iron group compared to control. The iron followed by Aeromonas hydrophila infection group caused a down-regulation in transferrin gene expression in all analyzed tissues at any point during the experiment. These findings demonstrate the evolutionary conservation of transferrin functions in vertebrates, involved in both the immune response and iron metabolism.</div>","PeriodicalId":37751,"journal":{"name":"Agri Gene","volume":"1 ","pages":"Pages 79-87"},"PeriodicalIF":0.0000,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aggene.2016.04.002","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agri Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352215116300095","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 2

Abstract

Transferrin (Tfa) plays an important role in iron regulation and is also known to be involved in response to infections. In this study, the full-length cDNA of the transferrin gene from Lutjanus argentiventris was cloned and characterized. The full-length cDNA of the tfa was 2482 bp encoding 690 amino acids containing an N-terminal signal peptide and the two conserved lobes. Tfa protein was highly similar to fish transferrins such as those from Larimichthys crocea (80%), Pagrus major (80%) and Dicentrarchus labrax (80%). Transferrin constitutive expression was found to be the highest in spleen and head-kidney. Moreover, the mRNA expression levels of tfa were measured by real-time PCR at 24 and 96 h in juveniles exposed to iron (Fe2x) during Aeromonas hydrophila infection. In general, the expression of tfa decreased in liver and intestine and increased in gill and skin in the iron group compared to control. The iron followed by Aeromonas hydrophila infection group caused a down-regulation in transferrin gene expression in all analyzed tissues at any point during the experiment. These findings demonstrate the evolutionary conservation of transferrin functions in vertebrates, involved in both the immune response and iron metabolism.

查看原文本刊更多论文

黄颡鱼(Lutjanus argentiventris)铁暴露及嗜水气单胞菌感染后转铁蛋白基因表达变化

转铁蛋白(Tfa)在铁调节中起重要作用，也参与对感染的反应。本研究克隆并鉴定了黄颡鱼转铁蛋白基因的全长cDNA。tfa全长2482 bp，编码690个氨基酸，包含1个n端信号肽和2个保守裂片。Tfa蛋白与Larimichthys crocea(80%)、Pagrus major(80%)和Dicentrarchus labrax(80%)的转铁蛋白高度相似。转铁蛋白组成表达在脾脏和头肾中最高。此外，通过实时荧光定量PCR检测了嗜水气单胞菌感染期间暴露于铁(Fe2x)的幼鱼在24和96 h时tfa mRNA的表达水平。总的来说，与对照组相比，铁组tfa在肝脏和肠道中的表达降低，在鳃和皮肤中的表达增加。嗜水气单胞菌感染组铁后，在实验的任何时刻，所有被分析组织的转铁蛋白基因表达均下调。这些发现证明了转铁蛋白在脊椎动物中功能的进化守恒，涉及免疫反应和铁代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

自引率

0.00%

发文量

期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.