Operational range for a continuous stirred-tank bioreactor degrading an inhibitory substrate

The Chemical Engineering Journal Pub Date : 1992-10-01 DOI:10.1016/0300-9467(92)80007-W

Cleophas L.C. Migiro, Wo̵dzimierz Sokół

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引用次数: 6

Abstract

An analysis of various models of a continuous stirred-tank bioreactor (CSTB) showed that those based on the specific uptake rate of substrate described the dynamics of the CSTB satisfactorily for controlling the parameters after a disturbance so that all substrate introduced into the bioreactor was utilized.

The maximum values of dilution rate D_max and/or substrate concentration S_{0 max} in the feed after a step increase in D and/or S₀ respectively which could be achieved without causing cell washout were measured and compared with those calculated using a “short-cut” method. It was found that the calculated values of D_max and S _{0 max} were lower by approximately 20% than the experimental results. Thus the values of D_max and S_{0 max} predicted by the short-cut method were not substantial underestimates and can be considered safe for the operation of a CSTB utilizing inhibitory substrates.

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降解抑制底物的连续搅拌槽生物反应器的操作范围

对连续搅拌槽生物反应器(CSTB)的各种模型进行了分析，结果表明，基于底物比吸收率的模型较好地描述了连续搅拌槽生物反应器(CSTB)的动力学特性，可以控制扰动后的参数，使所有引入的底物都被利用。分别测量D和/或S0逐步增加后可达到的饲料中稀释率Dmax和/或底物浓度S0 max的最大值，并与使用“捷径”方法计算的结果进行比较。结果表明，Dmax和s0 max的计算值比实验结果低约20%。因此，通过捷径方法预测的Dmax和S0 max值并没有实质性的低估，可以认为使用抑制底物的CSTB的操作是安全的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Chemical Engineering Journal

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