{"title":"Monoclonal Fab Fragments from Combinatorial Libraries Displayed on the Surface of Phage","authors":"Burton Dennis R., Barbas III Carlos F.","doi":"10.1006/immu.1993.1050","DOIUrl":null,"url":null,"abstract":"<div><p>The combinatorial antibody approach captures the immune repertoire within a library of bacteria or their viruses (phage). Current molecular biology techniques allow the construction of repertoires of a size that at least matches if not surpasses the size of the primary animal repertoire, about 10<sup>8</sup>. <em>Escherichia coli</em> are competent producers of antibody Fab and scFv fragments. To survey repertoires of this size for antibody fragments of the proper specificity and highest affinity, a phage display system that allows an affinity-based selection of clones was developed. These developments have at least three important consequences: (i) they allow direct cloning and expression of human Fab fragments; (ii) they allow the creation of synthetic antibodies; and (iii) they allow the <em>in vitro</em> evolution of antibody specificity and affinity. For the crystallographer, these developments will provide unique opportunities for the study of molecular recognition .c 1993</p></div>","PeriodicalId":79341,"journal":{"name":"ImmunoMethods","volume":"3 3","pages":"Pages 155-163"},"PeriodicalIF":0.0000,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/immu.1993.1050","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ImmunoMethods","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1058668783710508","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
The combinatorial antibody approach captures the immune repertoire within a library of bacteria or their viruses (phage). Current molecular biology techniques allow the construction of repertoires of a size that at least matches if not surpasses the size of the primary animal repertoire, about 108. Escherichia coli are competent producers of antibody Fab and scFv fragments. To survey repertoires of this size for antibody fragments of the proper specificity and highest affinity, a phage display system that allows an affinity-based selection of clones was developed. These developments have at least three important consequences: (i) they allow direct cloning and expression of human Fab fragments; (ii) they allow the creation of synthetic antibodies; and (iii) they allow the in vitro evolution of antibody specificity and affinity. For the crystallographer, these developments will provide unique opportunities for the study of molecular recognition .c 1993
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