L. C. Bekker, M. J. Bester, P. Hurter
{"title":"Reference steroid profiles in 0 to 3 day old neonates*","authors":"L. C. Bekker, M. J. Bester, P. Hurter","doi":"10.1002/mcs.1050","DOIUrl":null,"url":null,"abstract":"<p>The excretion of 16α-hydroxypregnenolone, 16α-hydroxy-DHEA, and 11-oxo-pregnanetriol was studied in 28 neonates with unambiguous genitalia. The 16α-hydroxypregnenolone:16α-hydroxy-DHEA ratio was determined and compared with the steroid ratios described by Shackleton and by Reynolds, and used to diagnose neonates with congenital adrenal hyperplasia. The technique used was capillary gas chromatography after solid phase extraction, enzymatic hydrolysis, and derivatization of the analytes. Duplicate analyses were performed to provide identification (mass selective detection–quadrupole ion trap) and quantification (flame ionization detection). © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 250–254, 2001</p>","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"13 6","pages":"250-254"},"PeriodicalIF":0.0000,"publicationDate":"2001-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/mcs.1050","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The journal of microcolumn separations : JMS","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mcs.1050","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
The excretion of 16α-hydroxypregnenolone, 16α-hydroxy-DHEA, and 11-oxo-pregnanetriol was studied in 28 neonates with unambiguous genitalia. The 16α-hydroxypregnenolone:16α-hydroxy-DHEA ratio was determined and compared with the steroid ratios described by Shackleton and by Reynolds, and used to diagnose neonates with congenital adrenal hyperplasia. The technique used was capillary gas chromatography after solid phase extraction, enzymatic hydrolysis, and derivatization of the analytes. Duplicate analyses were performed to provide identification (mass selective detection–quadrupole ion trap) and quantification (flame ionization detection). © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 250–254, 2001
0 ~ 3日龄新生儿的参考类固醇谱*
研究了28例具有明确生殖器的新生儿16α-羟基孕烯醇酮、16α-羟基脱氢表雄酮和11-氧-孕烯三醇的排泄情况。测定16α-羟基孕烯醇酮:16α-羟基脱氢表雄酮比值，并与Shackleton和Reynolds所描述的类固醇比值进行比较，用于诊断新生儿先天性肾上腺增生。所使用的技术是毛细管气相色谱法，经过固相萃取，酶解，衍生化分析物。进行重复分析以提供鉴定(质量选择检测-四极离子阱)和定量(火焰电离检测)。©2001 John Wiley &[J]微型电子，2001年9月13日:250-254
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