{"title":"Fluorimetric detection of cyclodextrins via complexation with fluorescence probe in microcolumn liquid chromatography","authors":"Toyohide Takeuchi, Yuko Kitamaki, Tomoo Miwa","doi":"10.1002/mcs.1014","DOIUrl":null,"url":null,"abstract":"<p>Cyclodextrins (CDs) are visualized by fluorimetric detection based on inclusion complexation with fluorescence probes in microcolumn liquid chromatography. Visualization of CDs is attributed to the fact that the fluorescence intensity of 2-<i>p</i>-toluidinylnaphthalene-6-sulfonate or other fluorescence probes increases when it is incorporated into the cavity of CDs. The present detection method can be applied to the determination of CDs contained in horseradish paste. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 19–23, 2001</p>","PeriodicalId":83120,"journal":{"name":"The journal of microcolumn separations : JMS","volume":"13 1","pages":"19-23"},"PeriodicalIF":0.0000,"publicationDate":"2001-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/mcs.1014","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The journal of microcolumn separations : JMS","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mcs.1014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Cyclodextrins (CDs) are visualized by fluorimetric detection based on inclusion complexation with fluorescence probes in microcolumn liquid chromatography. Visualization of CDs is attributed to the fact that the fluorescence intensity of 2-p-toluidinylnaphthalene-6-sulfonate or other fluorescence probes increases when it is incorporated into the cavity of CDs. The present detection method can be applied to the determination of CDs contained in horseradish paste. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 19–23, 2001
微柱液相色谱中荧光探针络合环糊精的荧光检测
采用微柱液相色谱法，利用荧光探针对环糊精(CDs)进行了包合检测。CDs的可视化归因于当2-对甲苯基萘-6-磺酸盐或其他荧光探针被纳入CDs的空腔时，其荧光强度增加。本方法可用于辣根膏中cd含量的测定。©2001 John Wiley &儿子公司J微型九月13:19-23,2001
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