Xing-Zheng Wu, Tiemin Huang, Wayne M. Mullett, Jupiter M. Yeung, Janusz Pawliszyn
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引用次数: 20
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Abstract
The recently developed whole-column imaged capillary isoelectric focusing is an advanced technique for the pI determination of proteins. In this study, we initially applied this technique to determine the pI of peanut allergens and its antibody, rabbit IgG. Two main proteins, Ara h I and Ara h II , were found in the peanut allergen solution. Their pI values were determined to be about 4.5–4.7 and 5.1–5.6, respectively. The value of pI for the antibody rabbit IgG was 6.1–6.5. Second, the immunoreaction selectivity of the two main peanut allergenic proteins with antibody rabbit IgG was studied. The CIEF results show that the peanut allergen Ara h II has a larger affinity for binding to the antibody rabbit IgG than Ara h I . © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 322–326, 2001
花生变应原蛋白-兔IgG抗体系统的等电点测定及免疫反应研究
近年来发展起来的全柱成像毛细管等电聚焦技术是一种测定蛋白质pI的先进技术。在本研究中，我们首次应用该技术测定花生过敏原及其抗体兔IgG的pI。在花生过敏原溶液中发现了两种主要蛋白质Ara h I和Ara h II。其pI值分别为4.5 ~ 4.7和5.1 ~ 5.6。兔IgG抗体的pI值为6.1 ~ 6.5。其次，研究两种主要花生致敏蛋白与兔抗体IgG的免疫反应选择性。CIEF结果表明花生过敏原Ara h II比Ara h i对兔抗体IgG的亲和力更大©2001 John Wiley &[J] .中国科技大学学报(自然科学版)，2001
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