{"title":"Thin Layer Chromatography","authors":"Li Cai","doi":"10.1002/9780470089941.et0603s08","DOIUrl":null,"url":null,"abstract":"<p>Thin layer chromatography (TLC) is a quick, sensitive, and inexpensive technique used to determine the number of components in a mixture, verify the identity and purity of a compound, monitor the progress of a reaction, determine the solvent composition for preparative separations, and analyze the fractions obtained from column chromatography. This unit is mainly aimed at novice experimenters, describing in detail the strategies and principal steps for performing a TLC experiment, with illustrations of the relevant instruments, as well as approaches for obtaining and understanding results. Valuable practical tips and troubleshooting solutions are also provided throughout the unit. <i>Curr. Protoc. Essential Lab. Tech</i>. 8:6.3.1-6.3.18. © 2014 by John Wiley & Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470089941.et0603s08","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780470089941.et0603s08","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 16
Abstract
Thin layer chromatography (TLC) is a quick, sensitive, and inexpensive technique used to determine the number of components in a mixture, verify the identity and purity of a compound, monitor the progress of a reaction, determine the solvent composition for preparative separations, and analyze the fractions obtained from column chromatography. This unit is mainly aimed at novice experimenters, describing in detail the strategies and principal steps for performing a TLC experiment, with illustrations of the relevant instruments, as well as approaches for obtaining and understanding results. Valuable practical tips and troubleshooting solutions are also provided throughout the unit. Curr. Protoc. Essential Lab. Tech. 8:6.3.1-6.3.18. © 2014 by John Wiley & Sons, Inc.
薄层色谱法
薄层色谱(TLC)是一种快速、灵敏、廉价的技术，用于确定混合物中成分的数量，验证化合物的特性和纯度，监测反应的进展，确定制备分离的溶剂组成，并分析从柱层析法获得的馏分。本单元主要针对实验新手，详细描述了进行TLC实验的策略和主要步骤，并附有相关仪器的插图，以及获取和理解结果的方法。宝贵的实用技巧和故障排除解决方案也提供了整个单位。咕咕叫。Protoc。基本的实验室。科技,8:6.3.1-6.3.18。©2014 by John Wiley &儿子,Inc。
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