Visualization of 5‐HT Receptors Using Radioligand‐Binding Autoradiography

Abstract

Described in this unit are techniques to visualize the majority of serotonin (5‐hydroxytryptamine, 5‐HT) receptor subtypes in sections of frozen brain tissue using receptor autoradiography. Protocols for brain extraction and sectioning, radioligand exposure, autoradiogram generation, and data quantification are provided, as are the optimal incubation conditions for the autoradiographic visualization of receptors using agonist and antagonist radioligands. © 2016 by John Wiley & Sons, Inc.