Measurement of Behavioral Taste Responses in Mice: Two‐Bottle Preference, Lickometer, and Conditioned Taste‐Aversion Tests

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2016-12-01 DOI:10.1002/cpmo.18

D. Gaillard, J. Stratford

引用次数: 17

Abstract

The natural like and dislike of foods based on taste is one of the most easily observed behaviors in animals. Animals eat palatable foods and reject aversive foods, which makes measurement of taste perception possible using various behavioral techniques. Three different methods to accurately measure taste behavior are described here. First, two‐bottle preference tests evaluate whether a taste compound (tastant) is preferred over water. Second, lickometer tests quantify the like and dislike for multiple concentrations of the same tastant or multiple tastants at the same time. Finally, conditioned taste aversion tests accurately determine the perceived taste threshold for palatable tastants. Together, these diverse methods enable researchers to observe and measure behavioral taste responses in mice to any tastant. © 2016 by John Wiley & Sons, Inc.

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小鼠味觉行为反应的测量:两瓶偏好、舔液计和条件味觉厌恶测试

基于味道的对食物的自然喜恶是动物最容易观察到的行为之一。动物吃美味的食物，拒绝讨厌的食物，这使得味觉的测量可以使用各种行为技术。这里描述了三种准确测量味觉行为的不同方法。首先，两瓶偏好测试评估一种味道化合物(尝味剂)是否比水更受欢迎。其次，舔液计测试量化了同一种品尝剂的多种浓度或同时多种品尝剂的喜欢和不喜欢。最后，条件厌恶味觉测试准确地确定了对美味的感知味觉阈值。总之，这些不同的方法使研究人员能够观察和测量小鼠对任何口味的行为味觉反应。©2016 by John Wiley & Sons, Inc。

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.