Norio Yamamoto, Manabu Ueda-Wakagi, Takuya Sato, Kengo Kawasaki, Keisuke Sawada, Kyuichi Kawabata, Mitsugu Akagawa, Hitoshi Ashida
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引用次数: 119
Abstract
Facilitative glucose uptake transport systems are ubiquitous in animal cells and are responsible for transporting glucose across cell surface membranes. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders such as myocardial ischemia, diabetes mellitus, and cancer. Detailed in this unit are laboratory methods for assessing glucose uptake into mammalian cells. The unit is divided into five sections: (1) a brief overview of glucose uptake assays in cultured cells; (2) a method for measuring glucose uptake using radiolabeled 3-O-methylglucose; (3) a method for measuring glucose uptake using radiolabeled 2-deoxyglucose (2DG); (4) a microplate method for measuring 2DG-uptake using an enzymatic, fluorometric assay; and (5) a microplate-based method using a fluorescent analog of 2DG. © 2015 by John Wiley & Sons, Inc.
培养细胞葡萄糖摄取的测定
促进性葡萄糖摄取运输系统在动物细胞中普遍存在，负责将葡萄糖运送到细胞膜表面。葡萄糖摄取的评估在许多疾病和代谢紊乱(如心肌缺血、糖尿病和癌症)的研究中至关重要。本单元详细介绍了评估哺乳动物细胞葡萄糖摄取的实验室方法。本单元分为五个部分:(1)培养细胞中葡萄糖摄取测定的简要概述;(2)使用放射性标记的3- o -甲基葡萄糖测量葡萄糖摄取的方法;(3)使用放射性标记的2-脱氧葡萄糖(2DG)测量葡萄糖摄取的方法;(4)利用酶促荧光法测定2dg摄取的微孔板法;(5)基于微孔板的方法，使用2DG的荧光模拟物。©2015 by John Wiley &儿子,Inc。
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