Caenorhabditis elegans as a Model for Toxic Effects of Nanoparticles: Lethality, Growth, and Reproduction

Laura L. Maurer, Ian T. Ryde, Xinyu Yang, Joel N. Meyer
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引用次数: 19

Abstract

The nematode Caenorhabditis elegans is extensively utilized in toxicity studies. C. elegans offers a high degree of homology with higher organisms, and its ease of use and relatively inexpensive maintenance have made it an attractive complement to mammalian and ecotoxicological models. C. elegans provides multiple benefits, including the opportunity to perform relatively high-throughput assays on whole organisms, a wide range of genetic tools permitting investigation of mechanisms and genetic sensitivity, and transparent bodies that facilitate toxicokinetic studies. This unit describes protocols for three nanotoxicity assays in C. elegans: lethality, growth, and reproduction. This unit focuses on how to use these well-established assays with nanoparticles, which are being produced in ever-increasing volume and exhibit physicochemical properties that require alteration of standard toxicity assays. These assays permit a broad phenotypic assessment of nanotoxicity in C. elegans, and, when used in combination with genetic tools and other assays, also permit mechanistic insight. © 2015 by John Wiley & Sons, Inc.

秀丽隐杆线虫作为纳米颗粒毒性效应的模型:致死性、生长和繁殖
秀丽隐杆线虫被广泛应用于毒性研究。秀丽隐杆线虫与高等生物具有高度的同源性,其易于使用和相对廉价的维护使其成为哺乳动物和生态毒理学模型的一个有吸引力的补充。秀丽隐杆线虫提供了多种好处,包括对整个生物体进行相对高通量分析的机会,允许调查机制和遗传敏感性的广泛遗传工具,以及促进毒性动力学研究的透明机构。本单元描述了秀丽隐杆线虫的三种纳米毒性测定方法:致死率、生长和繁殖。本单元的重点是如何使用这些成熟的纳米颗粒测定法,纳米颗粒的产量不断增加,并且表现出需要改变标准毒性测定法的物理化学性质。这些检测允许对秀丽隐杆线虫的纳米毒性进行广泛的表型评估,并且,当与遗传工具和其他检测结合使用时,也允许进行机制洞察。©2015 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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