Screening of Nutrients for Enrichment of Extracellular Pullulanase Production by Isolated Bacillus cereus KKSJ1981 Using Plackett–Burman Design

IF 1.2 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

National Academy Science Letters Pub Date : 2023-03-27 DOI:10.1007/s40009-023-01241-y

Jonna Siva Jyothi, Emandi Hemalatha, Tadikamalla Satish, Kadimpati Kishore Kumar

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Abstract

Current work intended to screen the different carbon, nitrogen sources and minerals which have a significant impact on the production of pullulanase by isolated Bacillus cereus KKSJ 1981 using Plackett–Burman design (PBD). Total 11 compounds (5 carbon sources, 3 nitrogen sources and 3 mineral salts) were screened by using 16 runs PBD. Each variable studied at 3 levels. The data were analyzed by first-order polynomial equation. The correlation coefficient was found to be 0.9876 and indicates the goodness of fit. Pareto chart of effects, normal probability plot and main effects plots were used to identify the significant variables and their level. It was observed that monosaccharide’s and dextran were found to be insignificant and remaining all variables found to be significant for production of pullulanase by isolated bacteria. Among all significant variables yeast extract has highest effect followed by soluble starch and MnSO₄. This study reveals that yeast extract at low concentration, soluble starch and MnSO₄ at higher level needed for the high titer of pullulanase production from B.cereus KKSJ 1981.

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用Plackett-Burman设计筛选蜡样芽孢杆菌KKSJ1981胞外普鲁兰酶生产富集的营养物质

采用Plackett-Burman设计(PBD)筛选对蜡样芽孢杆菌kksj1981产普鲁兰酶有重要影响的碳、氮源和矿物。通过16次PBD共筛选出11个化合物(5个碳源、3个氮源和3个矿物盐)。每个变量在3个水平上进行研究。采用一阶多项式方程对数据进行分析。相关系数为0.9876，表示拟合优度。使用帕累托效应图、正态概率图和主效应图来识别显著变量及其水平。观察到，单糖和葡聚糖被发现是不显著的，其余所有变量被发现对分离菌生产普鲁兰酶有显著影响。在所有显著变量中，酵母浸膏的影响最大，其次是可溶性淀粉和MnSO4。本研究表明，蜡样芽孢杆菌KKSJ 1981生产高效价的普鲁兰酶需要低浓度的酵母提取物、高水平的可溶性淀粉和MnSO4。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

National Academy Science Letters 综合性期刊-综合性期刊

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

12 months

期刊介绍： The National Academy Science Letters is published by the National Academy of Sciences, India, since 1978. The publication of this unique journal was started with a view to give quick and wide publicity to the innovations in all fields of science