Diet of deep-sea holothurians in theVolcanologists Massif, Bering sea, as inferred from stable isotope and fatty acid analyses

Abstract

Holothurians are one of the key components of the deep-sea megabenthos, often forming significant aggregations with high abundance and biomass. The crucial factors determining the pattern of their spatial distribution might be their food strategies, as well as the quantity and quality of available food. Using the set of trophic markers (analysis of fatty acid (FA) composition and stable nitrogen and carbon isotope signatures), we studied the trophic preferences of four common species of deep-sea holothurians (Paelopatides sp., Pannychia henrici, Psolidium sp., and Scotoplanes kurilensis) inhabiting the Volcanologists Massif, Bering Sea, at depths from 984 m to 3395 m. The δ¹³С values in tissues of all four species varied slightly (from −16.2 to −16.6‰) and were significantly higher than those in sediment organic matter (SOM) (on average, −21.5‰), which indicates the use of organic matter of the same origin by all species. The δ¹⁵N values (10.1–12.9‰) were also significantly higher than in SOM (on average, 4.4‰). The lowest average δ¹⁵N value was recorded for the deepest-dwelling species (Scotoplanes kurilensis), and the highest value from the shallowest-dwelling ones (Psolidium sp.). All the species differed significantly in FA composition. Psolidium sp. was distinguished by the highest saturated FA content (45.5%) and the lowest polyunsaturated FA (PUFA) content (18.7%). In the rest of the species, PUFAs dominated (>35%). The ratios of major PUFAs varied among the four species studied but all holothurians species were rich in phytoplankton derived FA suggesting tight trophic coupling to phytodetritus. However, for Psolidium sp., bacteria could constitute a substantial portion in the diet. The high level of 20:4n-6 might indicate a significant contribution of protozoans to the diet of Pannychia henrici. Paelopatides sp. and Scotoplanes kurilensis were characterized by a high level of 20:5n-3 and a high value of the 20:5n-3/20:4n-6 ratio, which indicates the predominance of fresh phytodetritus in their diet. The high level of primary production in the upper layers of the Bering Sea provides the high quality of the sedimentary OM and food supply of deposit feeders even at the greatest depths.