Protective effects of epigallocatechin-3-gallate counteracting the chronic hypobaric hypoxia-induced myocardial injury in plain-grown rats at high altitude.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cell Stress & Chaperones Pub Date : 2023-11-01 Epub Date: 2023-10-24 DOI:10.1007/s12192-023-01386-1
Haotian Chen, Chen Chen, Yuhui Qin, Lei Wang, Jie Zheng, Fabao Gao
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Abstract

Exposure to hypobaric hypoxia (HH) environment causes stress to the body, especially the oxygen-consuming organs. Chronic HH conditions have adverse effects on the myocardium. Thus, we conducted this experiment and aim to evaluate such adverse effects and explore the therapeutic role of epigallocatechin-3-gallate (EGCG) in rats' heart under chronic HH conditions. For that purpose, we transported rats from plain to a real HH environment at high altitude for establishing the HH model. At high altitude, animals were treated with EGCG while the salidroside was used as the positive control. General physiological data were collected, and routine blood test results were analyzed. Cardiac magnetic resonance (CMR) was examined to assess the structural and functional changes of the heart. Serum levels of cardiac enzymes and pro-inflammatory cytokines were examined. Oxidative markers in the left ventricle (LV) were detected. Additionally, ultrastructural and histopathological changes and apoptosis of the LV were assessed. Furthermore, the antioxidant stress-relevant proteins nuclear factor E2-related factor 2 (Nrf2) and the heme oxygenase-1 (HO-1) were detected. The experiment revealed that EGCG treatment decreased HH-induced elevation of cardiac enzymes and relieved mitochondrial damage of the LV. Notably, EGCG treatment significantly alleviated oxidative stress in the LV and inflammatory response in the blood. Western blot confirmed that EGCG significantly upregulated Nrf2 and HO-1. Therefore, EGCG may be considered a promising natural compound for treating the HH-induced myocardial injuries.

Abstract Image

表没食子儿茶素-3-没食子酸酯对高原平原生长大鼠慢性低压缺氧心肌损伤的保护作用。
暴露在低压缺氧(HH)环境中会对身体,尤其是耗氧器官造成压力。慢性HH对心肌有不良影响。因此,我们进行了这个实验,目的是评估这种不良反应,并探索表没食子儿茶素3-没食子酸盐(EGCG)在慢性HH条件下对大鼠心脏的治疗作用。为此,我们将大鼠从平原转移到高海拔的真实HH环境中,以建立HH模型。在高海拔地区,动物接受EGCG治疗,同时使用红景天苷作为阳性对照。收集一般生理数据,分析血常规检查结果。检查心脏磁共振(CMR)以评估心脏的结构和功能变化。检测血清心肌酶和促炎细胞因子水平。在左心室(LV)中检测到氧化标记物。此外,还评估了左心室的超微结构和组织病理学变化以及细胞凋亡。此外,还检测了抗氧化应激相关蛋白核因子E2相关因子2(Nrf2)和血红素加氧酶-1(HO-1)。实验表明,EGCG治疗降低了HH诱导的心肌酶升高,减轻了左心室的线粒体损伤。值得注意的是,EGCG处理显著减轻了左心房的氧化应激和血液中的炎症反应。蛋白质印迹证实EGCG显著上调Nrf2和HO-1。因此,EGCG可能被认为是治疗HH诱导的心肌损伤的一种有前景的天然化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Stress & Chaperones
Cell Stress & Chaperones 生物-细胞生物学
CiteScore
7.60
自引率
2.60%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.
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