5-HT7 receptors mediate dilation of rat cremaster muscle arterioles in vivo

IF 1.9 4区 医学 Q3 HEMATOLOGY
William F. Jackson, Armond Daci, Janice M. Thompson, Gregory D. Fink, Stephanie W. Watts
{"title":"5-HT7 receptors mediate dilation of rat cremaster muscle arterioles in vivo","authors":"William F. Jackson,&nbsp;Armond Daci,&nbsp;Janice M. Thompson,&nbsp;Gregory D. Fink,&nbsp;Stephanie W. Watts","doi":"10.1111/micc.12808","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>Serotonin (5-HT) infusion in vivo causes hypotension and a fall in total peripheral resistance. However, the vascular segment and the receptors that mediate this response remain in question. We hypothesized that 5-HT<sub>7</sub> receptors mediate arteriolar dilation to 5-HT in skeletal muscle microcirculation.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Cremaster muscles of isoflurane-anesthetized male Sprague-Dawley rats were prepared for in vivo microscopy of third- and fourth-order arterioles and superfused with physiological salt solution at 34°C. Quantitative real-time PCR (RT-PCR) was applied to pooled samples of first- to third-order cremaster arterioles (2–4 rats/sample) to evaluate 5-HT<sub>7</sub> receptor expression.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Topical 5-HT (1–10 nmols) or the 5-HT<sub>1/7</sub> receptor agonist, 5-carboxamidotryptamine (10–30 nM), dilated third- and fourth-order arterioles, responses that were abolished by 1 μM SB269970, a selective 5-HT<sub>7</sub> receptor antagonist. In contrast, dilation induced by the muscarinic agonist, methacholine (100 nmols) was not inhibited by SB269970. Serotonin (10 nmols) failed to dilate cremaster arterioles in 5-HT<sub>7</sub> receptor knockout rats whereas arterioles in wild-type litter mates dilated to 1 nmol 5-HT, a response blocked by 1 μM SB269970. Quantitative RT-PCR revealed that cremaster arterioles expressed mRNA for 5-HT<sub>7</sub> receptors.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>5-HT<sub>7</sub> receptors mediate dilation of small arterioles in skeletal muscle and likely contribute to 5-HT-induced hypotension, in vivo.</p>\n </section>\n </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/micc.12808","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microcirculation","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/micc.12808","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective

Serotonin (5-HT) infusion in vivo causes hypotension and a fall in total peripheral resistance. However, the vascular segment and the receptors that mediate this response remain in question. We hypothesized that 5-HT7 receptors mediate arteriolar dilation to 5-HT in skeletal muscle microcirculation.

Methods

Cremaster muscles of isoflurane-anesthetized male Sprague-Dawley rats were prepared for in vivo microscopy of third- and fourth-order arterioles and superfused with physiological salt solution at 34°C. Quantitative real-time PCR (RT-PCR) was applied to pooled samples of first- to third-order cremaster arterioles (2–4 rats/sample) to evaluate 5-HT7 receptor expression.

Results

Topical 5-HT (1–10 nmols) or the 5-HT1/7 receptor agonist, 5-carboxamidotryptamine (10–30 nM), dilated third- and fourth-order arterioles, responses that were abolished by 1 μM SB269970, a selective 5-HT7 receptor antagonist. In contrast, dilation induced by the muscarinic agonist, methacholine (100 nmols) was not inhibited by SB269970. Serotonin (10 nmols) failed to dilate cremaster arterioles in 5-HT7 receptor knockout rats whereas arterioles in wild-type litter mates dilated to 1 nmol 5-HT, a response blocked by 1 μM SB269970. Quantitative RT-PCR revealed that cremaster arterioles expressed mRNA for 5-HT7 receptors.

Conclusions

5-HT7 receptors mediate dilation of small arterioles in skeletal muscle and likely contribute to 5-HT-induced hypotension, in vivo.

Abstract Image

5-HT7受体介导大鼠提睾肌小动脉的扩张
目的5-羟色胺(5-HT)在体内输注可引起低血压和总外周阻力下降。然而,血管段和介导这种反应的受体仍然存在疑问。我们假设5-HT7受体介导骨骼肌微循环中小动脉扩张至5-HT。方法制备异氟烷麻醉雄性Sprague-Dawley大鼠的Cremaster肌,用于体内第三和第四级小动脉的显微镜检查,并在34°C下用生理盐水进行超泡。将定量实时PCR(RT-PCR)应用于一至三阶cremaster小动脉的合并样本(2-4只大鼠/样本),以评估5-HT7受体的表达。结果局部5-HT(1-10 nmols)或5-HT1/7受体激动剂5-甲酰胺色胺(10-30 nM),扩张的第三和第四级小动脉,1 μM SB269970,一种选择性5-HT7受体拮抗剂。相反,毒蕈碱激动剂甲基胆碱(100 nmols)不受SB269970的抑制。血清素(10 nmols)不能扩张5-HT7受体敲除大鼠的cremaster小动脉,而野生型窝仔的小动脉扩张到1 nmol 5-HT,1 μM SB269970。定量RT-PCR显示cremaster小动脉表达5-HT7受体的mRNA。结论5-HT7受体介导骨骼肌小动脉的扩张,并可能导致体内5-HT诱导的低血压。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Microcirculation
Microcirculation 医学-外周血管病
CiteScore
5.00
自引率
4.20%
发文量
43
审稿时长
6-12 weeks
期刊介绍: The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信