Isolation, characterization, mathematical modeling and assessment of a novel Photobacterium sp. EAH3, as toxicity biosensor

Abstract

Marine ecosystems are full of unique microbial phenomena, among them bioluminescence that could be harnessed for different ecological functions. This study was conducted to isolate luminous bacteria and utilize their glowing properties as a potential biosensor for toxicity assessment designs. It is worth noting that this study is the first report on the isolation of an Octopus associated Photobacterium species from Alexandria, Egypt. Based on light intensity, strain Photobacterium sp. EAH3 was selected and identified using 16S-rRNA gene sequencing. The luminescence production was further optimized using experimental. Among eight tested variables, the Plackett-Burman design showed that yeast extract, glycerol, peptone, and NaCl were the most important factors affecting luminescence. The central composite design calculated the optimum concentration of these variables in the optimized medium, which enhanced the bioluminescence activity by 1.39 folds. The employment of Photobacterium sp. EAH3 as a biosensor for the toxicity assessment of some solvents, hydrocarbons and heavy metals was examined using bioluminescence inhibition assay. According to the IC₅₀ results, the highest toxicity values recorded were 0.004 M, 4.15 ppm and 1.4 ppm for isoamyl alcohol, Hg²⁺ and catechol, respectively. Moreover, our study sheds light on futuristic methodology for monitoring toxicity in real polluted environmental and effluents' samples reflecting its promising efficiency.