Could alder buckthorn (Frangula alnus Mill) be a source of chemotherapeutics effective against hepato- and colorectal carcinoma? An in vitro study

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-10-14 DOI:10.1016/j.mrgentox.2023.503706

Stefana Vuletić , Marina Bekić , Sergej Tomić , Biljana Nikolić , Stefana Cvetković , Tea Ganić , Dragana Mitić-Ćulafić

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引用次数: 1

Abstract

Among numerous types of cancer, hepatocellular and colorectal carcinoma are important causes of mortality. Given the nature of these cancer types and their resistance, it is of great importance to find new chemotherapeutics and therapy targets, so plant products seem to be an excellent choice in such search. The main goal of this study was to investigate anticancer activity of Frangula alnus ethyl-acetate extract (FA) and its dominant constituent emodin (E) on hepatocellular and colorectal carcinoma cell lines, HepG2 and HCT116, as well as on normal MRC-5 fibroblasts. Cytotoxicity was investigated in MTT test and both FA and E showed strong reduction of cell viability in cancer cells. Flow cytometer analysis demonstrated that FA and E led to G1 phase arrest and slight accumulation of cells in the G2/M phase; additionally, annexinV-FITC/7AAD dying showed that FA and E decreased cell viability and triggered apoptosis in all cell lines. FA and E evidenced strong genotoxic potential in comet assay performed on all cell lines, while tests measuring antioxidative potential (DPPH and TBA) demonstrated strong effect of FA. It could be concluded that both FA and E have significant anticancer activity against hepatocellular and colorectal carcinoma cell lines HepG2 and HCT116, but notable selectivity was not observed.

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alder buckthorn（Frangula alnus Mill）是一种有效治疗肝癌和结直肠癌的化疗药物吗？体外研究

在众多类型的癌症中，肝细胞癌和结直肠癌是导致死亡的重要原因。鉴于这些癌症类型的性质及其耐药性，寻找新的化疗药物和治疗靶点非常重要，因此植物产品似乎是此类研究的绝佳选择。本研究的主要目的是研究番石榴乙酸乙酯提取物（FA）及其主要成分大黄素（E）对肝细胞和结直肠癌细胞系HepG2和HCT116以及正常MRC-5成纤维细胞的抗癌活性。MTT法检测细胞毒性，FA和E均显著降低癌症细胞的细胞活力。流式细胞仪分析表明，FA和E导致G1期阻滞，细胞在G2/M期轻微积聚；此外，annexinV FITC/7AAD染色显示FA和E降低了所有细胞系的细胞活力并引发细胞凋亡。在对所有细胞系进行的彗星试验中，FA和E表现出强大的遗传毒性潜力，而抗氧化潜力（DPPH和TBA）的测试显示FA具有强大的作用。可以得出结论，FA和E对肝细胞和结直肠癌细胞系HepG2和HCT116都具有显著的抗癌活性，但没有观察到显著的选择性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.