An immediate and antibody protected carboxyl quantum dot immunochromatographic analysis hierarchical signal amplification test strip based on biotin-streptavidin system for the detection of aflatoxin B1 in peanuts

Pub Date : 2023-10-10 DOI:10.1016/j.jfca.2023.105759

Bei Yuan , Qiang Liu , Qingqing Yang , Chengchen Pang , Haitao Xu , Xiaohui Du , Lin Wei , Kunying Nie , Yemin Guo , Xia Sun

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引用次数: 1

Abstract

As a relatively mature detection method, the test strip can quickly and sensitively detect the target. We designed an immunochromatographic strip based on biotin-streptavidin system to protect antibody (Ab) activity and achieve signal amplification. The coupling condition of biotin (B) and antibody is relatively mild, which protects the activity of antibody. The high affinity between biotin and streptavidin (SA) and the one-to-many binding mode can connect multiple quantum dots (QDs) together to achieve a certain signal amplification effect. The coupling conditions of biotin and antibody (B-Ab), the working volume of QDs-SA and the volume of B-Ab were optimized. Under the optimal conditions, aflatoxin B1 in peanut was detected. The detection range obtained by the experimental method was 1–10 μg/Kg, compared with the traditional quantum dot test strip method under the same conditions in this experiment, the amount of detection antibody is reduced and the detection range is increased. Compared with other immunochromatographic methods based on quantum dots to detect aflatoxin B1 (AFB1), the detection limit can reach the same order of magnitude, and the detection time is less.

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基于生物素-链霉亲和素系统的即时和抗体保护羧基量子点免疫色谱分析分级信号扩增试纸条检测花生中黄曲霉毒素B1

试纸条作为一种相对成熟的检测方法，能够快速灵敏地检测目标。我们设计了一种基于生物素-链霉亲和素系统的免疫层析条，以保护抗体（Ab）活性并实现信号放大。生物素（B）和抗体的偶联条件相对温和，保护了抗体的活性。生物素与链亲和素（SA）之间的高亲和力以及一对多结合模式可以将多个量子点连接在一起，从而达到一定的信号放大效果。优化了生物素与抗体（B-Ab）的偶联条件、量子点SA的工作体积和B-Ab的体积。在最佳条件下，对花生中的黄曲霉毒素B1进行了检测。该实验方法获得的检测范围为1–10μg/Kg，与本实验相同条件下的传统量子点试纸条法相比，检测抗体量减少，检测范围增加。与其他基于量子点的免疫层析方法检测黄曲霉毒素B1（AFB1）相比，检测极限可以达到相同数量级，检测时间更短。

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