Differences in virulence and oocyst shedding profiles in lambs experimentally infected with different isolates of Cryptosporidium parvum

IF 1.7 Q3 PARASITOLOGY
Paul M. Bartley , Sarah Thomson , Nicholas N. Jonsson , Alessandra Taroda , A. Innes Elisabeth , Frank Katzer
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引用次数: 1

Abstract

A wide spectrum of disease severity associated with cryptosporidiosis has been described, ranging from asymptomatic to fatal in both human and animal hosts. The reasons for the variations in severity are likely to be multifactorial, involving environmental, host and parasite factors. This paper describes two experimental infection trials in lambs, a symptomatic host for the parasite, to investigate variation in the clinical manifestations following infection with two distinct isolates of Cryptosporidium parvum. In the first experiment, groups of naïve lambs were challenged with one of two isolates (CP1 or CP2) at ​< ​1 week of age, to test the effect of the isolates on disease outcome. In a second experiment one group of lambs challenged at < 1 week of age (CP1) was then re-challenged with the same isolate at 6 weeks of age (CP1), while a second group was challenged for the first time at 6 weeks of age (CP1). This experiment examined age-related disease symptoms, oocyst shedding and the effect of prior exposure to the parasite on a subsequent homologous challenge. The two isolates were associated with significant differences in the demeanour of the animals and in the numbers of oocysts shed in the faeces. There were also differences in the duration and severity of diarrhoea, though these were not significant. The age of the lamb, at the time of a primary challenge (<1 week or 6 weeks), also resulted in differences in clinical outcomes, with younger lambs showing more severe clinical disease than the older lambs (feeding profiles and presentation of diarrhoea), while older lambs showed virtually no signs of infection but still produced large numbers of oocysts.

Abstract Image

小隐孢子虫不同分离株感染羔羊的毒力和卵囊脱落特征的差异
已经描述了与隐孢子虫病相关的广泛疾病严重程度,从人类和动物宿主的无症状到致命。严重程度变化的原因可能是多因素的,包括环境、宿主和寄生虫因素。本文描述了在羔羊身上进行的两项实验性感染试验,羔羊是该寄生虫的症状宿主,以研究感染两种不同的微小隐孢子虫分离株后临床表现的变化。在第一个实验中,在​<;​1周龄,以测试分离物对疾病结果的影响。在第二个实验中,一组羔羊在<;1周龄(CP1)在6周龄时用相同的隔离物(CP1。该实验检查了与年龄相关的疾病症状、卵囊脱落以及先前接触寄生虫对随后同源攻击的影响。这两种分离物在动物的行为举止和粪便中脱落的卵囊数量方面存在显著差异。腹泻的持续时间和严重程度也存在差异,尽管这些差异并不显著。初次挑战时羔羊的年龄(<1周或6周)也导致了临床结果的差异,年幼的羔羊比年长的羔羊表现出更严重的临床疾病(喂养特征和腹泻症状),而年长的羔羊几乎没有感染迹象,但仍产生大量卵囊。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
3.60
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