Colorimetric detection of phosphate-based on iron complexing catechol-displacement assay in eutrophicated water bodies

Pub Date : 2023-02-09 DOI:10.1016/j.enmm.2023.100792

Rafaqat Naveen , Abdul Niaz , Muhammad Iqbal Zaman , Shahanzeb Khan , Abdur Rahim , Asma Zulfiqar , Muhammad Tariq , Tawfik. A. Saleh , Diogo M. F. Santos , Mabkhoot Alsaiari

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Abstract

A simple, sensitive, and inexpensive colorimetric indicator-displacement assay based on Fe³⁺-catechol complex has been developed to determine PO₄^3-. The Fe³⁺ and catechol formed a greenish color complex, upon the addition of PO₄^3- to the complex, it exhibited a visual color change immediately from greenish to colorless along with UV–vis spectral change. This change was mainly attributed to the high binding affinity of PO₄^3- with Fe³⁺ by displacing catechol from the Fe³⁺-catechol complex. The developed sensor showed good sensitivity for the determination of PO₄^3- in a wide linear range from 2 − 1000 µM with a limit of detection (LOD) of 0.5 µM which was found to be lower than the permissible limit. The sensor presented good selectivity towards the detection of PO₄^3- in the existence of other potential interfering anions. Furthermore, the method was successfully applied to determine PO₄^3- in eutrophicated water bodies (river and lake water) with good recoveries and without showing any significant matrix effect.

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富营养化水体中基于铁络合儿茶酚置换法的磷酸盐比色检测

建立了一种简单、灵敏、廉价的基于Fe3+-邻苯二酚络合物的比色指示剂置换法测定PO43-。Fe3+和邻苯二酚形成了一种绿色复合物，在复合物中加入PO43-后，随着紫外-可见光谱的变化，其视觉颜色立即从绿色变为无色。这种变化主要归因于PO43-通过从Fe3+-邻苯二酚络合物中取代邻苯二酚而与Fe3+具有高结合亲和力。所开发的传感器在2−1000µM的宽线性范围内对PO43的测定显示出良好的灵敏度，检测限（LOD）为0.5µM，低于允许限。在存在其他潜在干扰阴离子的情况下，该传感器对PO43-的检测具有良好的选择性。此外，该方法已成功应用于富营养化水体（河水和湖水）中PO43-的测定，回收率高，没有显示出任何显著的基质效应。

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