Molecular and morphometric study of Brazilian populations of Psychodopygus davisi

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY
Bruno Leite Rodrigues, Glaucilene da Silva Costa, Rodrigo Espíndola Godoy, Antonio Marques Pereira Júnior, Wilsandrei Cella, Gabriel Eduardo Melim Ferreira, Jansen Fernandes de Medeiros, Paloma Helena Fernandes Shimabukuro
{"title":"Molecular and morphometric study of Brazilian populations of Psychodopygus davisi","authors":"Bruno Leite Rodrigues,&nbsp;Glaucilene da Silva Costa,&nbsp;Rodrigo Espíndola Godoy,&nbsp;Antonio Marques Pereira Júnior,&nbsp;Wilsandrei Cella,&nbsp;Gabriel Eduardo Melim Ferreira,&nbsp;Jansen Fernandes de Medeiros,&nbsp;Paloma Helena Fernandes Shimabukuro","doi":"10.1111/mve.12701","DOIUrl":null,"url":null,"abstract":"<p>In this study, we analysed the molecular and morphometric differences of several populations of the putative sand fly vector <i>Psychodopygus davisi</i> (Root, 1934) (Diptera, Psychodidae, Phlebotominae) in Brazil. We amplified the 658 base pair fragments of the DNA barcoding region—cytochrome <i>c</i> oxidase subunit 1 (<i>COI</i>) gene—for 57 specimens of <i>P. davisi</i> and three specimens of <i>Psychodopygus claustrei</i> (Abonnenc, Léger &amp; Fauran, 1979). We merged our data with public sequences of the same species available from GenBank. Then, the combined dataset—87 sequences and 20 localities—was analysed using population structure analysis and different species delimitation approaches. Geometric morphometry of wings was performed for 155 specimens of <i>P. davisi</i> populations from the North, Midwest and Southeast Brazilian regions, analysing the differences in centroid sizes and canonical variates. Molecular analysis indicated high intraspecific genetic distance values for <i>P. davisi</i> (maximum <i>p</i> distance = 5.52%). All algorithms identified <i>P. davisi</i> and <i>P. claustrei</i> as distinct molecular taxonomic units, despite the low interspecific distance (<i>p</i> distance to the nearest neighbour = 4.79%). <i>P. davisi</i> sequences were split into four genetic clusters by population structure analysis and at least five genetic lineages using intermediate scenarios of the species delimitation algorithms. The species validation analysis of BPP strongly supported the five-species model in our dataset. We found high genetic diversity in this taxon, which is in agreement with its wide geographic distribution in Brazil. Furthermore, the wing analysis showed that specimens from the Southeast Region of Brazil are different from those in the North and the Midwest. The evolutionary patterns of <i>P. davisi</i> populations in Brazil suggest the presence of candidate species, which need to be validated in future studies using a more comprehensive approach with both genomic data and morphological characters.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical and Veterinary Entomology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/mve.12701","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

In this study, we analysed the molecular and morphometric differences of several populations of the putative sand fly vector Psychodopygus davisi (Root, 1934) (Diptera, Psychodidae, Phlebotominae) in Brazil. We amplified the 658 base pair fragments of the DNA barcoding region—cytochrome c oxidase subunit 1 (COI) gene—for 57 specimens of P. davisi and three specimens of Psychodopygus claustrei (Abonnenc, Léger & Fauran, 1979). We merged our data with public sequences of the same species available from GenBank. Then, the combined dataset—87 sequences and 20 localities—was analysed using population structure analysis and different species delimitation approaches. Geometric morphometry of wings was performed for 155 specimens of P. davisi populations from the North, Midwest and Southeast Brazilian regions, analysing the differences in centroid sizes and canonical variates. Molecular analysis indicated high intraspecific genetic distance values for P. davisi (maximum p distance = 5.52%). All algorithms identified P. davisi and P. claustrei as distinct molecular taxonomic units, despite the low interspecific distance (p distance to the nearest neighbour = 4.79%). P. davisi sequences were split into four genetic clusters by population structure analysis and at least five genetic lineages using intermediate scenarios of the species delimitation algorithms. The species validation analysis of BPP strongly supported the five-species model in our dataset. We found high genetic diversity in this taxon, which is in agreement with its wide geographic distribution in Brazil. Furthermore, the wing analysis showed that specimens from the Southeast Region of Brazil are different from those in the North and the Midwest. The evolutionary patterns of P. davisi populations in Brazil suggest the presence of candidate species, which need to be validated in future studies using a more comprehensive approach with both genomic data and morphological characters.

Abstract Image

Abstract Image

巴西戴维斯灵虫种群的分子和形态计量学研究。
在这项研究中,我们分析了巴西假定的沙蝇媒介Psychodopygus davisi(Root,1934)(双翅目,Psychodidae,Phlebotominae)几个种群的分子和形态计量学差异。我们扩增了57份戴维斯P.davisi和3份克劳斯特雷精神病虫标本的DNA条形码区细胞色素c氧化酶亚基1(COI)基因的658个碱基对片段(Abonnenc,Léger&Fauran,1979)。我们将我们的数据与GenBank提供的相同物种的公开序列合并。然后,使用种群结构分析和不同的物种划界方法对组合数据集87个序列和20个地点进行了分析。对来自巴西北部、中西部和东南部地区的155个达维西种群的翅膀进行了几何形态测量,分析了质心大小和典型变量的差异。分子分析表明,山竹具有较高的种内遗传距离值(最大P距离 = 5.52%)。所有算法都将P.davisi和P.clausstrei确定为不同的分子分类单元,尽管种间距离(与最近邻居的P距离)较低 = 4.79%)。通过种群结构分析将P.davisi序列划分为四个遗传簇和至少五个遗传谱系。BPP的物种验证分析有力地支持了我们数据集中的五个物种模型。我们在这个分类单元中发现了高度的遗传多样性,这与其在巴西的广泛地理分布相一致。此外,翅膀分析表明,来自巴西东南部地区的标本与北部和中西部的标本不同。巴西山竹种群的进化模式表明存在候选物种,这需要在未来的研究中使用更全面的方法,结合基因组数据和形态特征进行验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信