Differential gene expression patterns in Sorghum bicolor genotypes in response to high vapor pressure deficit

IF 1 Q3 AGRONOMY

Journal of Crop Improvement Pub Date : 2022-09-03 DOI:10.1080/15427528.2021.2009077

M. Machingura, Dave Rajvi, Alysa Rodriguez, Z. Pan, J. Bajsa-Hirschel

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Abstract

ABSTRACT The ability of land plants to partially close their stomata in response to high vapor pressure deficit (VPD), called the limited transpiration trait, is a rare phenomenon in plants. The characteristic has been demonstrated in several crop species, including Sorghum bicolor. Genotypes within the sorghum species respond in different ways to high VPD, but the molecular basis and regulatory mechanisms of the responses are not clear. This study was conducted to determine the basis for the differences in stomatal responses to high VPD in sorghum genotypes (SC1345, SC35, and Macia). Gene expression patterns of genes involved in the abscisic acid pathway were investigated using quantitative-PCR after exposing plants to high VPD. The results showed marked differences in expression patterns for the genes across the three genotypes, coupled to differences in abscisic acid content. The results suggested natural variation in abscisic acid content within sorghum. Expression patterns in the genes BG1, ZEP, and SDR, in addition to the NCED3 gene, provide a basis for the variations in stomatal responses to high VPD in this crop species.

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高粱双色基因型对高蒸气压亏空反应的差异基因表达模式

摘要陆地植物在高蒸气压不足（VPD）的情况下部分关闭气孔的能力，被称为有限蒸腾特性，在植物中是一种罕见的现象。这种特性已经在几种作物中得到证实，包括双色高粱。高粱物种的基因型对高VPD有不同的反应，但反应的分子基础和调节机制尚不清楚。本研究旨在确定高粱基因型（SC1345、SC35和Macia）对高VPD的气孔反应差异的基础。在植物暴露于高VPD后，使用定量PCR研究了参与脱落酸途径的基因的基因表达模式。结果显示，三种基因型的基因表达模式存在显著差异，脱落酸含量也存在差异。结果表明高粱中脱落酸含量存在自然变异。除了NCED3基因外，BG1、ZEP和SDR基因的表达模式为该作物物种对高VPD的气孔反应变化提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Crop Improvement Multiple-

CiteScore

3.30

自引率

7.70%

发文量

期刊介绍： Journal of Crop Science and Biotechnology (JCSB) is a peer-reviewed international journal published four times a year. JCSB publishes novel and advanced original research articles on topics related to the production science of field crops and resource plants, including cropping systems, sustainable agriculture, environmental change, post-harvest management, biodiversity, crop improvement, and recent advances in physiology and molecular biology. Also covered are related subjects in a wide range of sciences such as the ecological and physiological aspects of crop production and genetic, breeding, and biotechnological approaches for crop improvement.