Isonzyme Changes and DNA Damage Associated with Sterility by Gamma Radiation in Trogoderma granarium Everts Males

IF 0.4 4区农林科学 Q4 ENTOMOLOGY

Entomological News Pub Date : 2021-12-09 DOI:10.3157/021.130.0107

R. Sayed, Nilly A. H. Abdelfattah

引用次数: 0

Abstract

ABSTRACT: The present study aimed to investigate the impact of gamma radiation on isoenzymes (peroxidase and polyphenyl oxidase) and DNA damage in Trogoderma granarium. The newly emerged male adults were irradiated with substerilizing and sterilizing doses of gamma radiation for the experiments (100 and 200Gy). The results showed that there was no difference in the distribution of peroxidase and polyphenyl oxidase isoenzymes, and the intensity of peroxidase isoenzymes was the same between the tested samples when compared to the control population and each other. There was a variation in the intensity of the detected polyphenyl oxidase isoenzyme. The DNA electrophoresis showed fragmentation in the gamma irradiated adults as compared to the control. Therefore, it could be concluded that these parameters could be used as a biomarker for identification of gamma irradiated T. granarium males.

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γ射线辐照麦雄性不育的同工酶变化及DNA损伤

摘要：本研究旨在研究γ辐射对仓毛滴虫同工酶（过氧化物酶和聚苯氧化酶）和DNA损伤的影响。对新出现的成年男性进行了亚消毒和灭菌剂量的伽马辐射照射，用于实验（100和200Gy）。结果表明，过氧化物酶和聚苯氧化酶同工酶的分布没有差异，与对照群体相比，受试样品的过氧化物酶同工酶强度相同。检测到的聚苯氧化酶同工酶的强度存在变化。DNA电泳显示，与对照组相比，γ射线照射的成年人中出现了碎片。因此，可以得出结论，这些参数可以用作鉴定γ辐照的粮仓T.granarium雄性的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Entomological News 生物-昆虫学

CiteScore

1.10

自引率

25.00%

发文量

期刊介绍： Contributions are accepted on any of the aspects of systematics and taxonomy of insects. This includes: taxonomic revisions, phylogenetics, biogeography and faunistics, and descriptive morphology of insects. Taxonomic papers describing single species are acceptable if such a study is sufficiently justified, however, comprehensive studies that provide a comparative, integrated study of insect systematics and taxonomy will get priority.