COMPARISON BETWEEN BIOMETRIC AND ELECTROLYTE LEAKAGE TESTS AS INDICATORS OF MAIZE SEED VIGOR

L. C. Pereira, L. V. Correia, David Fernando Posso Suárez, C. Carvalho, A. Braccini
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Abstract

The objective of this work was to compare the accuracy of seedling tests based on electrolyte release to distinguish different maize seed vigor levels with the accuracy provided by biometric tests for seedling length and biomass. For this purpose, seeds with different vigor levels of the Garra Viptera hybrid had their physiological potential evaluated through tests of germination, accelerated aging, seed electrical conductivity, field emergence, seedling length, seedling dry biomass, seedling electrical conductivity, potassium leakage and membrane injury index. Completely randomized design was used, with four replications, except for the field emergence variable, when the randomized complete block design was adopted. The obtained means were compared by Fisher's LSD test, at 5% probability, and then Pearson’s correlation coefficients were calculated between the tests based on seedling performance and the physiological quality results obtained with the seeds. In addition to their lower sensitivity to istinguish the different vigor levels, the biometric tests (length and biomass) showed values of correlation with field emergence considerably lower than those btained with the membrane permeability tests (seedling electrical conductivity, otassium leakage and membrane injury index). Among the tests based on electrolyte extravasation, the membrane injury index was the most accurate to differentiate maize seed lots in different vigor levels, as well as to estimate the seed emergence in the field.
玉米种子活力指标生物测定法与电解质渗漏法的比较
这项工作的目的是将基于电解质释放来区分不同玉米种子活力水平的幼苗测试的准确性与生物特征测试提供的幼苗长度和生物量的准确性进行比较。为此,通过发芽、加速老化、种子电导率、田间出苗、幼苗长度、幼苗干生物量、幼苗电导率、漏钾量和膜损伤指数等测试,对不同活力水平的夜蛾夜蛾种子的生理潜力进行了评价。采用完全随机区组设计时,除田间出苗变量外,采用完全随机设计,共四次重复。以5%的概率通过Fisher的LSD检验对获得的平均值进行比较,然后根据幼苗性能和种子获得的生理质量结果计算测试之间的Pearson相关系数。除了对区分不同活力水平的敏感性较低外,生物特征测试(长度和生物量)显示与田间出苗的相关性值远低于膜渗透测试(幼苗电导率、钾渗漏和膜损伤指数)。在基于电解质外渗的测试中,膜损伤指数最准确地区分不同活力水平的玉米种子批次,并评估种子在田间的出苗情况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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