Insights into the catalytic mechanism of ligninolytic peroxidase and laccase in lignin degradation

IF 1.9 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Bioremediation Journal Pub Date : 2022-03-08 DOI:10.1080/10889868.2021.1973951

P. Bhatt, M. Tiwari, Prasoon Parmarick, Kalpana Bhatt, S. Gangola, Muhammad Adnan, Yashpal Singh, M. Bilal, Shakeel Ahmed, Shaohua Chen

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引用次数: 8

Abstract

Abstract The present study aimed to give insights into the binding interactions of the laccases and peroxidase for the degradation of the lignin. The active site of the applied enzymes contains the amino acids that are playing an essential role in lignin degradation. The binding pocket amino acids have interacted with the lignin via the hydrogen, alkyl and van der Waal bonds. The mutagenesis in the active sites of these enzymes predicted the increasing and decreasing performance of the lignin-degrading enzymes. Ramachandran plot analysis of the Laccase and peroxidases determined the active conformation of the lignin-degrading enzymes. Phylogenetic study of the Laccase, lignin peroxidase, versatile peroxidase and manganese peroxidase suggested each of the enzymes belong to the separate protein cluster. The present study reveals the binding potential of the various lignin-degrading enzymes that could increase our understanding of the application of plant biomass large scales.

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木质素分解过氧化物酶和漆酶对木质素降解的催化机理探讨

摘要本研究旨在深入了解漆酶和过氧化物酶在降解木质素中的结合相互作用。应用酶的活性位点包含在木质素降解中起重要作用的氨基酸。结合口袋氨基酸通过氢键、烷基键和范德华键与木质素相互作用。这些酶活性位点的突变预测了木质素降解酶性能的提高和降低。漆酶和过氧化物酶的Ramachandran图分析确定了木质素降解酶的活性构象。漆酶、木质素过氧化物酶、多功能过氧化物酶和锰过氧化物酶的系统发育研究表明，每种酶都属于单独的蛋白质簇。本研究揭示了各种木质素降解酶的结合潜力，这可以增加我们对植物生物质大规模应用的理解。

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来源期刊

Bioremediation Journal ENVIRONMENTAL SCIENCES-

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

9 months

期刊介绍： Bioremediation Journal is a peer-reviewed quarterly that publishes current, original laboratory and field research in bioremediation, the use of biological and supporting physical treatments to treat contaminated soil and groundwater. The journal rapidly disseminates new information on emerging and maturing bioremediation technologies and integrates scientific research and engineering practices. The authors, editors, and readers are scientists, field engineers, site remediation managers, and regulatory experts from the academic, industrial, and government sectors worldwide. High-quality, original articles make up the primary content. Other contributions are technical notes, short communications, and occasional invited review articles.