Single nucleotide polymorphism, haplotypes and genotypes of Mannose-binding lectin (MBL1) gene and their association with clinical mastitis in Murrah buffalo

IF 0.2 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE
S. A. Shergojry, A. Verma, I. D. Gupta, N. A. Mir
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引用次数: 0

Abstract

The Mannose-binding lectin (MBL1) gene is an important component of immune response system. It plays vital role in activation of the complement system and act as chief defense molecule of host cell to provide protection against various diseases.  In the recent study, six novel single nucleotide polymorphisms (SNPs), at 2689G>C, 2751A>G, 4822T>C, 4853A>G, 4855T>C, and 4978T>C resulted in 2 non synonymous type of change at 4853A>G, 4855T>C resulting in one amino acid substitution Ser150Gly of MBL1 protein and their association with clinical mastitis were investigated in two hundred (200) Murrah buffaloes. These SNPs in MBL1 were genotyped by using the polymerase chain reaction (PCR) and Deoxyribose nucleic acid (DNA) sequencing methods in order to reveal the association with clinical mastitis. SHEsis software tool was used for construction of haplotypes and Linkage disequilibrium analysis. Twenty one (21) haplotypes were constructed. Allelic association analysis showed that C allele of 2689 G>C, A allele of 2751 A>G, C allele of 4822 T>C, A allele of 4853 A>G, C allele of 4855 T>C, and C allele of 4978 T>C had significant association with increased risk of clinical mastitis in Murrah buffaloes (P<0.05). Murrah buffaloes with CG genotype of 2689 G>C, AG or GG genotype of 2751 A>G, CC genotype of 4822T>C, AG or GG genotype of 4853 A>G, TC or CC genotype of 4855 T>C and CC genotype of 4978 T>C loci had significantly lower incidence of clinical mastitis compared to their counter genotypes. Haplotype association analysis showed that Hap21 (TATTGA) was found significantly related to higher risk of clinical mastitis (P<0.05). Hap14 (TGTTGG) and Hap5 (CGCCCA) were found significantly associated with lower risk of clinical mastitis in Murrah buffalo (P<0.05).
Murrah水牛甘露聚糖结合凝集素(MBL1)基因单核苷酸多态性、单倍型、基因型及其与临床乳腺炎的关系
甘露糖结合凝集素(MBL1)基因是免疫应答系统的重要组成部分。它在补体系统的激活中起着至关重要的作用,并作为宿主细胞的主要防御分子,为抵御各种疾病提供保护。在最近的研究中,在200头Murrah水牛中,研究了2689G>C、2751A>G、4822T>C、4853A>G、4855T>C和4978T>C的6个新的单核苷酸多态性(SNPs)导致4853A>G4855T>C的2个非同义型变化,导致MBL1蛋白的一个氨基酸取代Ser150Gly,以及它们与临床乳腺炎的关系。利用聚合酶链式反应(PCR)和脱氧核糖核酸(DNA)测序方法对MBL1中的这些SNPs进行基因分型,以揭示其与临床乳腺炎的关系。SHEsis软件工具用于单倍型的构建和连锁不平衡分析。构建了二十一(21)个单倍型。等位基因关联分析表明,2689G>C的C等位基因、2751A>G的A等位基因,4978T>C的C等位基因与Murrah水牛临床乳腺炎风险增加显著相关(2751A>G的PC、AG或GG基因型、4822T>C的CC基因型、4853A>G的AG或GG型、4855T>C的TC或CC基因型和4978T>C基因型临床乳腺炎的发病率与反基因型相比显著降低。单倍型关联分析显示,Hap21(TATTGA)与临床乳腺病的高风险显著相关(P<0.05)。Hap14(TGTTGG)和Hap5(CGCCCA)与Murrah水牛临床乳腺炎的低风险显著相关(P<0.05)。
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来源期刊
Buffalo Bulletin
Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
0.50
自引率
0.00%
发文量
53
审稿时长
>12 weeks
期刊介绍: Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.
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