Histological and Histochemical Description of the Pancreas in the Adult Lesser Capybara (Hydrochoerus isthmius)

Gabriel Álvarez Otero, Cesar Rojano Bolaño, Juan Carrascal Velásquez
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引用次数: 0

Abstract

The aim of this study was to describe histologically and histochemically the pancreas of three adult Hydrochoerus isthmius, attacked by feral dog in Buenavista, Córdoba, Colombia. A complete necropsy was performed and pancreatic fragments were collected and stored in 10% buffered formalin and Bouin liquid. Then, they were dehydrated, diaphanized and embedded in paraffin, stained with Hematoxylin & Eosin, P.A.S., Gomori trichrome, Grimelius and Masson Fontana modified. The H. isthmius pancreas presented a duodenal mesenteric pattern. The exocrine portion was described as a composite tubuloacinar gland. The endocrine portion of the pancreas was constituted by pancreatic islets of Langerhans and a diffuse neuroendocrine system. The histological and histochemical techniques used allowed us to identify the exocrine and endocrine portion of the organ. It is suggested to complement this study with some special techniques for the identification of specific endocrine cells, such as Beta, Alpha, Delta, Epsilon, PP or Gamma.
成年小水豚胰腺的组织学和组织化学描述
本研究的目的是从组织学和组织化学的角度描述哥伦比亚科尔多瓦州布埃纳维斯塔市三只被野狗袭击的成年水螅的胰腺。进行完全尸检,收集胰腺碎片并储存在10%缓冲福尔马林和Bouin液体中。然后,将它们脱水、透明化并包埋在石蜡中,用苏木精和曙红、P.A.S.、Gomori三色染色法、Grimelius和Masson Fontana修饰法染色。H.峡部胰腺呈现十二指肠-肠系膜模式。外分泌部分被描述为一个复合的管-脑腺。胰腺的内分泌部分由朗格汉斯的胰岛和弥漫性神经内分泌系统组成。所使用的组织学和组织化学技术使我们能够识别器官的外分泌和内分泌部分。建议用一些特殊的技术来补充这项研究,以鉴定特定的内分泌细胞,如Beta、Alpha、Delta、Epsilon、PP或Gamma。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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审稿时长
16 weeks
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