Experimental study of tropism of cultivated canine parvovirus in the immunogenesis organs of puppies

IF 0.5 Q4 BIOLOGY
M. Radzykhovskyi, I. Sokulskiy, O. Dyshkant, A. Antoniuk, B. Gutyj, R. Sachuk
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引用次数: 1

Abstract

The immune system unites the organs and the tissues that protect the organism against genetically alien cells or substances entering the organism from the environment. Canine parvovirus is an etiologic agent of hemorrhagic gastroenteritis and causes a significant problem for veterinary medicine due to high level of morbidity and mortality, mostly among dogs, because of fast progression without immune-complement response. In this study, based on the results of our clinical, virological, histological, histochemical and morphological assays, we determined the pathogenetic role of parvovirus in sick dogs experimentally infected per os, specifically with isolated canine parvovirus (Antaeus) with titer of infectious activity equaling 3.80 ± 0.008 lg TCID50/cm, cultivated on heterological cell cultures. This allowed us to clarify, add to and generalize the data on the pathogenesis of the disease and determine pathohistological and histochemical changes in the immunogenesis, since the studied virus expresses immune-suppressive properties, leading to ruination of the locomotor ability of the organism and fast lethal outcome. The study of pathomorphological changes was carried out using pathoanatomical and histologic methods. Pathoanatomical material from the autopsy of puppies aged 45 days was fixed in 10% aqueous solution of neutral formaline and embedded in paraffin. Having parvoviral infection, dogs experience pathomorphologic changes in immune-complement organs, indicating inhibition of the immunogenesis function during an infectious disease of viral etiology. In the immunogenesis organs of puppies with the experimental reconstruction of parvoviral enteritis, we microscopically determined the following: edema of the cortex and medulla, disorganization of thymic corpuscules, and impairment of processes of differentiation of lymphocytes in the cortex and medulla of the thymic lobules; edema and large areas of accumulation of hemosiderin in the spleen as a result of breakdown of large amount of erythrocytes; acute inflammatory hyperemia of the parenchyma, swelling of sinuses, serous and serous-hemorrhagic lymphadenitis in lymph nodes. The complex of histologic changes in the immune protection organs, which we found in the conditions of experimental reconstruction of parvoviral infection, can be considered a distinct criterion for pathomorphologic differentiation diagnostics of parvoviral enteritis in dogs.
犬细小病毒在幼犬免疫发生器官中向性的实验研究
免疫系统将保护生物体免受从环境中进入生物体的遗传外来细胞或物质侵害的器官和组织结合在一起。犬细小病毒是出血性肠胃炎的病原体,由于发病率和死亡率高,主要是在狗中,由于进展迅速而没有免疫补体反应,因此给兽医带来了重大问题。在本研究中,基于我们的临床、病毒学、组织学、组织化学和形态学分析结果,我们确定了细小病毒在实验感染peros的病犬中的致病作用,特别是用在异源细胞培养物上培养的感染活性滴度为3.80±0.008lg TCID50/cm的分离的犬细小病毒(Antaeus)。这使我们能够澄清、补充和推广有关该疾病发病机制的数据,并确定免疫发生中的病理组织学和组织化学变化,因为所研究的病毒表达免疫抑制特性,导致机体运动能力的破坏和快速致死的结果。采用病理解剖学和组织学方法对病理形态学变化进行研究。将45天大幼犬尸检的病理解剖材料固定在10%中性甲酰胺水溶液中,并包埋在石蜡中。犬感染细小病毒后,免疫补体器官发生病理形态学变化,表明在病毒病因的传染病中免疫发生功能受到抑制。在实验性细小病毒肠炎重建幼犬的免疫原性器官中,我们在显微镜下确定了以下内容:皮质和髓质水肿、胸腺小体紊乱以及胸腺小叶皮质和髓质淋巴细胞分化过程受损;由于大量红细胞的分解,脾脏出现水肿和大面积含铁血黄素积聚;急性炎性实质充血,鼻窦肿胀,淋巴结浆液性和浆液性出血性淋巴结炎。我们在细小病毒感染的实验重建条件下发现的免疫保护器官的复杂组织学变化,可以被认为是犬细小病毒肠炎病理形态学鉴别诊断的一个独特标准。
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来源期刊
CiteScore
0.90
自引率
0.00%
发文量
25
审稿时长
10 weeks
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