Criopreservación de semen canino (Canis familiaris) en pajilla francesa en el municipio de Medellín, Antioquia

Abstract

The objective of this work was to apply the canine semen cryopreservation methodology in French straw in Medellin-Antioquia. A seminal collection was carried out to eight adult canines, by gloved hand method, each ejaculate was assessed macroscopically and microscopically and subsequently diluted with Triladyl® with a final concentration of 80x106 sperm/ml. Semen samples were packed in 0.5 ml straws and a quick-freezing process was performed (nitrogen vapors for 25 minutes and then transferred directly to liquid nitrogen, french straw method). In fresh semen, a milky whitish appearance, a volume of 2.27 ml (± 1.40), a concentration of 388.5x106 sperm/mL (± 228.069), individual motility of 79% (± 4%) and vigor of 3.96 (± 0.327) were observed. 123 straws were obtained, of which 27 were taken randomly and individual motility (51 ± 19%) and vigor (2.89 ± 1.02) were evaluated. In conclusion, the application of the methodology of quick-freezing by french straw, using Triladyl® as a cryoprotectant, is suggested as an efficient protocol for cryopreservation of canine semen in Medellin-Antioquia.