Chromosomal mapping of ribosomal clusters and telomeric sequences (TTAGG) n in nine species of lobsters (Crustacea, Decapoda)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-06-12 DOI:10.1080/24750263.2023.2217188

S. Salvadori, F. Deidda, L. Carugati, R. Melis, E. Costa, M. Sibiriu, E. Coluccia

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Abstract

Abstract Lobsters are ubiquitous, economically important decapod crustaceans with apparently conflicting evolutionary relationships. Here, we describe the chromosomal location of the major (45S rDNA) and minor (5S rDNA) ribosomal gene families in four species of Astacidea and five of Achelata, using two-color FISH. The major ribosomal family is located in 4–16 sites per diploid chromosome set, with Nephrops norvegicus (Nephropidae) showing the highest number described so far in Decapoda. The 5S rDNA is located in two sites in eight species; only in the crayfish Procambarus clarkii the 5S FISH signals were detected in four sites together with additional weaker signals. Furthermore, in N. norvegicus the minor ribosomal genes are syntenic with one major ribosomal cluster. Moreover, we located by two-color FISH the pentanucleotide (TTAGG) n telomeric repeat in the Nephropidae studied, showing the occurrence of a colocalization with 45S ribosomal sequences in Homarus gammarus. The comparison of chromosomal locations of repetitive sequences in Mediterranean, Atlantic, and South African lobster species as well as in marine and freshwater ones provides information on chromosomal evolution and cytotaxonomy of Decapoda.

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九种龙虾（甲壳纲、十足目）核糖体簇和端粒序列（TTAGG）n的染色体定位

摘要龙虾是普遍存在的、具有重要经济意义的十足目甲壳类动物，其进化关系显然存在冲突。在这里，我们使用双色FISH描述了四种Astacida和五种Achelata的主要（45S rDNA）和次要（5S rDNA）核糖体基因家族的染色体位置。主要的核糖体家族位于每个二倍体染色体组的4-16个位点，其中褐皮肾科（Nephrops norvegicus）在十足目中显示出迄今为止最高的数量。5S rDNA位于8个物种的2个位点；只有在克氏原螯虾中，在四个位点检测到5S FISH信号以及其他较弱的信号。此外，在褐家鼠中，次要的核糖体基因与一个主要的核糖体簇是同基因的。此外，我们通过双色FISH定位了所研究的肾蛛科中的五核苷酸（TTAGG）n端粒重复序列，显示了与非洲猴45S核糖体序列的共定位。地中海、大西洋和南非龙虾物种以及海洋和淡水龙虾物种重复序列的染色体位置比较提供了十足目染色体进化和细胞分类学的信息。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.