The Study of Interaction Activity of Nickel (ll) Phthalocyanine Complex Bearing Tetra Substituted Phenoxy-3-Methoxybenzoic Acid Groups with DNA

Abstract

Nickel phthalocyanine complex containing 4-(3,4-dicyanophenoxy)-3-methoxybenzoic acid group was synthesized and specified by way of FT-IR, NMR, UV/Vis procedures. The binding of PcNi complex to CT-DNA was examined via electronic absorption titration, emission titration, melting temperature, viscosity measurement, and agarose gel electrophoresis technics, respectively. The DNA interaction activity of PcNi against CT-DNA was studied by way of UV/Vi titrations, fluorescence spectra, farther by conducting melting point, viscosity procedures in the buffer of a pH 7.02. The obtained outcomes from these methods demonstrated that PcNi indicated substantial binding affinity to the DNA via intercalating by the binding constant of 1.31 x 106 m-1. Further, the interaction activity of the complex on CT-DNA was investigated by which the electrophoresis technique and this procedure indicated that PcNi complex exhibits strong binding affinity on the DNA.