Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-05-30 DOI:10.20450/mjcce.2022.2238

Una-Jovana Vajić, J. Živković, M. Ivanov, D. Jovović, K. Šavikin, B. Bugarski, N. Mihailović-Stanojević

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Abstract

The aim of this study was to optimize the parameters of the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous-methanolic extracts had higher total flavonoid content than aqueous-ethanolic extracts. So, full factorial design and response surface methodology (RSM) were employed to estimate the effects of methanol content (50, 75, and 100 %) and extraction time (30, 60, and 90 minutes) on total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (Ferric Reducing Antioxidant Power Assay) antioxidant capacity, while extraction time had no significant effect on either of the responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous-methanol and 67 min, and 65 % aqueous-methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.

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响应面法优化刺荨麻叶中抗氧化剂的提取工艺

本研究旨在优化刺荨麻叶总黄酮的提取工艺参数。不同溶剂对总黄酮含量影响的比较表明，无论提取时间如何，甲醇水提物的总黄酮含量均高于乙醇水提物。因此，采用全因子设计和响应面法（RSM）来估计甲醇含量（50%、75%和100%）和提取时间（30、60和90分钟）对提取物总黄酮含量和抗氧化能力的影响。RSM分析表明，溶剂中甲醇含量显著影响总黄酮含量和FRAP（铁还原抗氧化能力测定）抗氧化能力，而提取时间对这两种反应均无显著影响。最大总黄酮含量的提取参数估计为69%的甲醇水溶液和67分钟，最大FRAP抗氧化能力的提取参数为65%的甲醇水混合物和83分钟。DPPH（2,2-二苯基-1-苦基肼基）的抗氧化能力不受萃取时间或溶剂中甲醇百分比的显著影响。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.