Impact Of Microbial Synergism On Second Generation Production Of Bioethanol From Fruit Peels Wastes

K. Mahmoud, S. Fahim
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Abstract

Fruit peels annually accumulate in huge amounts; fruit wastes are rich in lignocellulosic component which can be recovered into mono simple carbohydrates able to utilize for bioethanol production. The enzymatic hydrolysis of lignocelluloses is known to be a key to the second-generation biofuel, the challenge is the still expensive enzymes involved in the saccharification process, loss of the most hemicellulose pentose sugars which were non-fermentable as base, beside the presence of hampers lignin thus require to resolve its problem. As part of study, followed the cost-effective means for bioethanol producing from four fruit peels (Banana, Orange, Mango and Watermelon), yielding of enzymes from co-fungal cultivation carried by Aspergillus niger and Phanerochaete chrysosporium, followed by co-fermentation of the saccharified sugars using yeast belong to Saccharomyces cerevisiae and Kluyveromyces marxianus which contributed for bioethanol production in 8 L lab-scale reactor. The fermented sugars recovering was reached to 27.77 g.l-1 from banana peels which were found to be good exploited as potential raw source, co-fungal enzymatic hydrolysis followed by yeast co-fermentation led to substantial yield by 10.74 g.l-1, the adding of calcium oxide increased the purity which leads finally to 97.5 wt % of pure bioethanol. Thrust towards fossil fuels replacement with renewable clean fuels such as bioethanol by using fruit peels residuals which are considered renewable energy source may also help in CO2 mitigation.
微生物协同作用对果皮废料第二代生物乙醇生产的影响
果皮每年积累大量;水果废弃物富含木质纤维素成分,可回收为可用于生物乙醇生产的单一简单碳水化合物。众所周知,木质纤维素的酶促水解是第二代生物燃料的关键,挑战在于糖化过程中涉及的酶仍然昂贵,除了存在阻碍木质素之外,失去了大多数不能发酵的半纤维素戊糖作为碱,因此需要解决其问题。作为研究的一部分,遵循从四种果皮(香蕉、橙子、芒果和西瓜)生产生物乙醇的成本效益方法,从黑曲霉和黄孢原毛平革菌携带的共真菌培养中生产酶,然后使用属于酿酒酵母(Saccharomyces cerevisiae)和马氏克鲁维酵母(Kluyveromyces marxianus)的酵母共同发酵糖化糖,这有助于在8L实验室规模的反应器中生产生物乙醇。从香蕉皮中回收的发酵糖达到27.77 g.l-1,这是一个很好的潜在原料来源,共真菌酶水解和酵母共发酵使产量达到10.74 g.l-1。添加氧化钙提高了纯度,最终获得97.5 wt%的纯生物乙醇。通过使用被视为可再生能源的果皮残渣,用生物乙醇等可再生清洁燃料取代化石燃料,也可能有助于缓解二氧化碳排放。
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