Optimization of Antioxidant and Antimicrobial Activities by Fractionation of Artemisia judaica subsp. sahariensis Crude Extract from Ahaggar (Algerian Arid Region): UPLC-ESI-MS/MS Analysis of Fractions
R. Kebbab, Aida basseddik, Walid Boussebaa, E. Nabti, K. Houali
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引用次数: 0
Abstract
In recent years, medicinal plants have received considerable attention due to the search for novel bioactive compounds. In this optic, we have been interested in Artemisia judaica subsp. Sahariensis, a Saharan species widely used in phytotherapy by the Tuaregs of the Ahaggar.
This study aims to evaluate and optimize the biological activities of this plant in order to valorize its bioactive compounds.
For this purpose, an extraction with methanol (70%) was carried out, then a liquid-liquid fractionation, using solvents with increasing polarity: diethyl ether, ethyl acetate, n-butanol and water. We realized an analysis of phenols, flavonoids and evaluation of antioxidant and antimicrobial activities in addition to the UPLC-ESI-MS/MS analysis of the fractions.
The reducing effect was proportional to the solvent polarity. The crude extract gave the best reducing power (17.55±3.06µg/mL), better phenols and flavonoids contents (20.35±0.5 mgGAE/gTDM), (10.35±0.56 mgGAE/gTDM) respectively compared to its fractions. The DPPH (2,2-Diphenyl-1-Picrylhydrazyl) radical scavenging assay showed that the ethyl acetate fraction was the most active with the lowest IC50 value (inhibitory concentration to 50% of DPPH) (41.43±0.24 µg/ml) followed by n-butanol (58.53±0.20 µg/mL), diethyl ether (135.07±6.18 µg/mL) and aqueous (226.41±1.51 µg/mL) fractions (p<0.0001). Moreover, hydromethanolic extract gave an IC50 value of 114.05±3.37 µg/mL. The antimicrobial effect was observed on all clinical multi-resistant bacteria tested except Klebsiella pneumoniae, which was resistant. The most important effect was observed by the ethyl acetate fraction against the fungal strain Candida albicans. Various phenolic acids and flavonoids (flavones, flavonols, flavanones) were detected and could be responsible for these bioactivities.
We can conclude that liquid-liquid extraction with solvents of increasing polarity plays a major role in optimizing the biological activity of this plant, which contains polyphenols and can therefore be valued as a source of natural antioxidants and antimicrobials.
Current Bioactive CompoundsPharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.90
自引率
0.00%
发文量
112
期刊介绍:
The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.