Evaluación del tiempo en la recuperación de larvas de nematodos a partir de un coprocultivo

Andrea María Leandro-Picado, Alexander Calderón-Sancho, Ramsés Alfaro-Mora
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Abstract

Gastrointestinal nematode infections represent a problem for the health of cattle, as well as affecting the economics of farmers and producing industries. The necessary conditions for the development of the eggs of the nematodes, contained in the feces of the bovine, were simulated using a coproculture in sawdust of laurel (Laurus nobilis). In this investigation it was tried to evaluate differences in the recovery time of nematode larvae from the coproculture. In the methodology, the flotation technique was used to identify nematode eggs and the McMaster to count the amount of eggs per gram of feces and the amount of larvae recovered from the coproculture at different times. The analysis of the results was performed with the Minitab software version 19, and an ANOVA was applied with a post hoc analysis of Tukey. It was established that exist a significant difference in the nematode count when they are collected at 3 hours.
从共育线虫中恢复幼虫的时间评估
胃肠道线虫感染对牛的健康构成了一个问题,也影响了农民和生产行业的经济。利用月桂(Laurus nobilis)木屑中的共培养物模拟了牛粪便中线虫卵发育的必要条件。在本研究中,试图评估线虫幼虫从粪菌中恢复时间的差异。在该方法中,使用浮选技术鉴定线虫卵,并使用麦克马斯特计数每克粪便中的卵数和不同时间从粪化石中回收的幼虫数。使用Minitab软件版本19进行结果分析,并将ANOVA应用于Tukey的事后分析。已经确定,当在3小时收集线虫时,线虫计数存在显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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