In vitro growth of nine edible ectomycorrhizal fungi under a range of pH conditions

IF 0.6 4区农林科学 Q4 AGRONOMY

Bioagro Pub Date : 2023-05-01 DOI:10.51372/bioagro352.8

Jaime Olaizola, O. Santamaría, J. Diez

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Abstract

Ectomycorrhizal fungi are considered to play an essential role in the development of forest ecosystems and can protect plant against pathogenic infections. Among other factors, soil pH may affect the successful inoculation of forest seedlings in nurseries. The effect of pH on the growth rate of strains of nine species of edible ectomycorrhizal (ECM) fungi was evaluated in vitro. In the experiments, Boletus edulis, B. aereus, B. pinophilus, B. fragrans, Amanita rubescens, Xerocomus ferrugineus, Lactarius deliciosus, Lactarius sanguifluus and Suillus luteus were grown in Petri dishes containing modified Melin Norkrans medium and adjusted at seven different pH levels. Colony area was measured at 7-day intervals for 8 weeks. Final fungal biomass and residual pH of the medium at 8th week were also measured. The optimum pH levels and pH tolerance ranges for the tested ECM fungal species are presented and discussed in the text. The results showed that the greatest growth in vitro was produced by A. rubescens and S. luteus at high pH levels (between 6.5-8.5), and by X. ferrugineus at low pH (3.5-6.5). Almost all the strains acidified the medium where they were grown after eight incubation weeks.

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9种可食用外生菌根真菌在不同pH条件下的体外生长

外生菌根真菌被认为在森林生态系统的发展中发挥着重要作用，可以保护植物免受病原感染。除其他因素外，土壤pH值可能影响苗圃中林木幼苗的成功接种。研究了pH对9种食用外生菌根（ECM）真菌生长速率的影响。在实验中，在含有改良Melin-Norkrans培养基的皮氏培养皿中培养了美味牛肝菌、aereus牛肝菌（B.aereus）、皮诺杆菌（B.pinophilus。每隔7天测量菌落面积，持续8周。还测量了第8周培养基的最终真菌生物量和残留pH。文中介绍并讨论了所测试ECM真菌物种的最佳pH水平和pH耐受范围。结果表明，在高pH（6.5-8.5）条件下，冬凌草和木犀草在体外生长最快，而在低pH（3.5-6.5）下，铁芽孢生长最快。几乎所有菌株在培养8周后都对其生长的培养基进行了酸化。

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来源期刊

Bioagro Agricultural and Biological Sciences-General Agricultural and Biological Sciences

CiteScore

1.40

自引率

37.50%

发文量

期刊介绍： Bioagro es una revista científica del Decanato de Agronomía de la Universidad Centroccidental “Lisandro Alvarado” (UCLA). Su periodicidad es cuatrimestral y se publica en los meses de enero, mayo y septiembre. Cada trabajo es revisado por al menos dos especialistas en el área, externos a la revista, de cuya opinión depende la aceptación definitiva. Se utiliza sistema de arbitraje doble ciego. La revista va dirigida, fundamental pero no exclusivamente, a profesionales y técnicos del área agrícola. Su objetivo es publicar trabajos científicos originales e inéditos en ciencias agrícolas que enfoquen aspectos de agronomía, botánica y propagación de plantas, entomología y zoología, suelos, fitopatología y protección vegetal, ingeniería agrícola, genética y mejoramiento de plantas, ecología, procesamiento de productos agrícolas, biotecnología y sociales. También pueden ser publicados artículos cortos en los que se presenten descubrimientos científicos, desarrollos tecnológicos y resultados de diagnósticos integrales, en la modalidad de Notas Técnicas. En Venezuela, se encuentra en las bibliotecas de todas las universidades e institutos de educación superior que ofrecen carreras agronómicas, así como de los entes oficiales de investigación agropecuaria. En el exterior, la revista llega a universidades y/o institutos de investigación agrícolas de todos los países de América Latina así como Estados Unidos, Canadá y España.