Aktivitas Antibakteri dan Skrining Fitokimia Ekstrak Etanol Daun Jambu Mete Anacardium occidentale L. Terhadap Pertumbuhan Escherichia coli Antibacterial Activity and Phytochemical Screening of Ethanol Extract of Cashew Leaves Anacardium occidentale L. Against Escherichia coli

MedULA Pub Date : 2022-11-08 DOI:10.46496/medula.v10i1.26112

E. Astuty

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引用次数: 1

Abstract

ABSTRACTBackground: All parts of the cashew tree Anacardium occidentale L. (especially the leaves and bark) have been widely used as traditional herbal medicines, and are used throughout the world. Thus, in recent decades, the traditional medicinal properties of the Anacardium plant, and its various biological effects are being studied extensively. Purpose(s): This study aims to conduct a preliminary study of the antibacterial properties and phytochemical screening of the ethanol extract of cashew leaves. Methods: The antibacterial test of was determined by the Kirby baeur disc paper diffusion method. Qualitative test of phytochemical content was carried out using chemical reagents. Results: The results indicated that the ethanol extract of cashew leaves has an inhibitory effect on the growth of Escherichia coli. The smallest inhibition zone was found in the extract with a concentration of 10% of 5.75 mm and the largest was found at a concentration of 100% with a diameter of 14 mm. The phytochemical screening revealed that extracts contained; alkaloids, flavonoids, steroids, terpenoids, phenolics, saponins, and tanins Conclusion: The ethanol extract of cashew leaves was able to inhibit the growth of Escherichia coli test bacteria at all concentration variations. Phytochemical screening also found that the ethanol extract of cashew leaves contains alkaloids, flavonoids, steroid terpenoids, phenolics, saponins, and tannins which are known to have antibacterial properties.Keywords: cashew, ethanol extract, antibacteria ABSTRAKLatar Belakang: Semua bagian pohon jambu mete Anacardium occidentale L. (terutama daun dan kulit batang) telah banyak digunakan sebagai obat herbal tradisional, dan digunakan di seluruh dunia. Dengan demikian, dalam beberapa dekade terakhir, sifat obat tradisional tanaman Anacardium, dan berbagai efek biologis sedang dipelajari secara ekstensif Tujuan: Penelitian ini bertujuan untuk melakukan studi pendahuluan sifat antibakteri dan skrining fitokimia dari ekstrak etanol daun jambu mete. Metode: Uji antibakteri ekstrak ditentukan dengan metode difusi kertas cakram Kirby baeur. Uji kualitatif kandungan fitokimia dalam ekstrak dilakukan dengan pereaksi kimia. Hasil: Ekstrak etanol daun jambu mete memiliki daya hambat terhadap pertumbuhan Escherichia coli. Zona hambat terkecil terdapat pada ekstrak dengan konsentrasi 10% sebesar 5,75 mm dan terbesar terdapat pada konsentrasi 100% dengan diameter 14 mm. Hasil skrining fitokimia menunjukkan bahwa ekstrak etanol daun jambu mete mengandung senyawa golongan alkaloid, flavonoid, steroid, terpenoid, fenolik, saponin, dan tanin Simpulan: Ekstrak etanol daun jambu mete mampu menghambat pertumbuhan bakteri uji Escherichia coli pada semua variasi konsentrasi. Skrining fitokimia juga menemukan bahwa dalam ekstrak etanol daun jambu mete mengandung alkaloid, flavonoid, steroid terpenoid, fenolik, saponin, dan tannin yang diketahui memiki kemampuan sebagai antibakteriKata Kunci: jambu mete, ekstrak etanol, antibakteri

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腰果叶乙醇提取物对大肠杆菌的抗菌活性和植物化学筛选

摘要背景：腰果的所有部位（尤其是叶和树皮）都被广泛用作传统中药材，并在世界各地广泛使用。因此，近几十年来，人们对Anacardium植物的传统药用特性及其各种生物学效应进行了广泛的研究。目的：本研究旨在对腰果叶乙醇提取物的抗菌性能和植物化学筛选进行初步研究。方法：采用Kirby-baeur纸片扩散法测定其抗菌活性。用化学试剂对植物化学成分进行了定性检测。结果：腰果叶乙醇提取物对大肠杆菌的生长有抑制作用。在浓度为10%的5.75mm提取物中发现最小的抑制区，在浓度为100%的直径为14mm的提取物中发现最大的抑制区。植物化学筛选显示提取物含有生物碱、黄酮类化合物、类固醇、萜类化合物、酚类化合物、皂苷类化合物和tanins类化合物结论：腰果叶乙醇提取物在各种浓度变化下都能抑制大肠杆菌试验菌的生长。植物化学筛选还发现，腰果叶的乙醇提取物含有生物碱、类黄酮、类固醇萜类、酚类、皂苷和单宁，这些物质已知具有抗菌特性。关键词：腰果、乙醇提取物、抗菌药[UNK]ABSTRAKLatar:西洋Anacardium occidentale L.的所有部位（尤其是叶和茎皮）都被广泛用作传统草药，并在世界范围内使用。因此，在过去的几十年里，人们对传统植物药Anacardium的性质及其各种生物学效应进行了广泛的研究。目的：本研究旨在对乙醇叶提取物进行抗菌前体研究和植物化学筛选。方法：采用Kirby-Baeur纸片扩散法测定提取物的抗菌性。提取物中植物化学成分的质量检测是通过化学作用进行的。结果：甲基蜡叶乙醇提取物对大肠杆菌生长有一定的抑制作用。最小的屏障区位于浓度为10%5.75mm的提取物上，最大的屏障区在直径为14mm的浓度为100%的提取物上。mete蠕虫的乙醇叶提取物可以在各种浓度下减缓大肠杆菌测试细菌的生长。植物化学筛选还发现，在乙醇提取物中，甲基炭疽叶中含有生物碱、类黄酮、萜类类固醇、酚类、皂苷和单宁，已知具有抗菌能力关键字：甲基炭疽，乙醇提取物，抗菌

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MedULA MEDICINE, GENERAL & INTERNAL-

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