The Mechanism of Propofol on Non-Small Cell Lung Cancer (NSCLC) through Modulating Mesenchymal Transition (EMT)

Asploro journal of biomedical and clinical case reports Pub Date : 2022-08-08 DOI:10.36502/2022/asjbccr.6272

Yi Yang, Yiding Zuo, Li Zhou

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Abstract

Background: Intensive investigations have focused on the effect of propofol on the malignant behaviours of cancer cells. However, much is still unknown about the effect of propofol on non-small cell lung cancer (NSCLC). Here we aimed to investigate the effect of propofol on NSCLC with cancer stem-like cells (CSCs) A549. Methods: CCK-8 assays, flow cytometry, and transwell assay were used to assess the changes in the proliferation, migration, and invasion in A549 treated with propofol. By detecting hallmarks of mesenchymal transition (EMT), the mechanism of the effect of propofol on A549 was assessed. Results: In A549, propofol exposure promoted cell proliferation, while inhibiting migration and invasion. By activating EMT using TGF-β pretreatment, propofol treatment downregulated hallmarks of EMT and led to inactivation of EMT. Conclusion: Modulation of self-renewal capacity of CSCs by anesthetics may affect cancer malignant behaviors following surgery. The employment of propofol not only exerts inhibitory effects on cancer cells but also on CSCs in non-small cell lung cancer.

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异丙酚通过调节间充质转移（EMT）对非小细胞肺癌癌症（NSCLC）的作用机制

背景：研究了异丙酚对癌症细胞恶性行为的影响。然而，关于异丙酚对癌症（NSCLC）的影响仍有许多未知之处。本文旨在研究异丙酚对癌症干细胞（CSCs）A549 NSCLC的影响。方法：采用CCK-8法、流式细胞术和transwell法检测异丙酚对A549细胞增殖、迁移和侵袭的影响。通过检测间充质转化（EMT）的特征，评估丙泊酚对A549的作用机制。结果：在A549细胞中，丙泊酚暴露促进细胞增殖，同时抑制迁移和侵袭。通过TGF-β预处理激活EMT，异丙酚治疗降低了EMT的标志并导致EMT失活。结论：麻醉剂对CSCs自我更新能力的调节可能影响癌症术后的恶性行为。异丙酚不仅对癌症细胞有抑制作用，而且对癌症非小细胞肺癌CSCs也有抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Asploro journal of biomedical and clinical case reports

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