A HPLC-UV METHOD FOR ANALYSIS OF TOTAL PLANT EXTRACT AND CATECHIN FRACTION OF BANCHA GREEN TEA

IF 0.7 Q4 CHEMISTRY, MEDICINAL

Natural Products Journal Pub Date : 2022-05-12 DOI:10.2174/2210315512666220512212448

S. Stoeva, Maya Radeva-Ilieva, I. Zhelev, K. Georgiev

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引用次数: 1

Abstract

The pharmacological characterization of Bancha green tea requires a preliminary establishment of its phytochemical composition. The aim of the present study was to develop a simple, sensitive, and environmentally friendly HPLC-UV procedure for the simultaneous quantification of caffeine, (+)-catechin, epigallocatechin gallate and gallic acid in different Bancha green tea isolates. The chromatographic separation of the four phytochemicals was performed on a Hypersil GOLD aQ column with a mobile phase consisting of acetonitrile/acetic acid/bi-distilled water (8:1:91 v/v/v), in an isocratic mode of elution. The suitability of the proposed procedure was verified by achieved satisfying linearity and specificity. The method was also assessed as accurate and precise, with the obtained limits of quantification of caffeine, (+)-catechin, epigallocatechin gallate, and gallic acid of 0.01, 0.30, 0.50 and 0.03 μg/mL, respectively. As it was expected, the analytical procedure approbation showed the highest epigallocatechin gallate content (16.78 % ± 0.54) in the catechin fraction. On the other hand, the amount of caffeine and epigallocatechin gallate in the total plant extract was almost the same (4.19 % ± 0.14 and 4.12 % ± 0.44, respectively). A reliable HPLC-UV method for phytochemical characterisation of Bancha green tea extracts has been developed. The conducted quantitate assay could be used as a basis for subsequent pharmacological in vivo and in vitro studies of Bancha green tea.

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高效液相色谱-紫外分光光度法测定板茶绿茶总植物提取物和儿茶素组分

Bancha绿茶的药理特性需要初步确定其植物化学成分。本研究的目的是开发一种简单、灵敏、环保的HPLC-UV方法，同时定量不同Bancha绿茶分离株中的咖啡因、（+）-儿茶素、表没食子儿茶素没食子酸盐和没食子酸。四种植物化学物质的色谱分离在Hypersil GOLD aQ柱上进行，流动相由乙腈/乙酸/双蒸馏水（8:1:91v/v/v）组成，以等度洗脱模式进行。通过获得令人满意的线性和特异性，验证了所提出的程序的适用性。该方法也被评估为准确无误，咖啡因、（+）-儿茶素、表没食子儿茶素没食子酸盐和没食子酸的定量限分别为0.01、0.30、0.50和0.03μg/mL。正如预期的那样，分析程序的认可显示儿茶素组分中表没食子儿茶素没食子酸盐含量最高（16.78%±0.54）。另一方面，总植物提取物中咖啡因和表没食子儿茶素没食子酸盐的含量几乎相同（分别为4.19%±0.14和4.12%±0.44）。所进行的定量分析可作为Bancha绿茶后续体内外药理学研究的基础。

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来源期刊

Natural Products Journal CHEMISTRY, MEDICINAL-

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： The Natural Products Journal a peer reviewed journal, aims to publish all the latest and outstanding developments in natural products. The Natural Products Journal publishes original research articles, full-length/mini reviews, letters and guest edited issues on all aspects of research and development in the field including: isolation, purification, structure elucidation, synthesis and bioactivity of chemical compounds found in nature.