GENOME SIZE DETERMINATION OF CUCUMBER (CUCUMIS SATIVUS), HONEYDEW (CUCUMIS MELO INODORUS) AND ROCK MELON (CUCUMIS MELO CANTALUPENSIS) VIA FLOW CYTOMETRY

Science Heritage Journal Pub Date : 2021-08-17 DOI:10.26480/gws.01.2021.14.16

Raden Muhamad Imaduddin Yumni, M. F. Karim, Mohd Razik Midin

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引用次数: 5

Abstract

The family of Cucurbitaceae consists of species with economical and nutritional value. Morphologically, there are only few differences between Cucumis species. The interspecific and intraspecific variation in the genome size of the Cucumis species are not discovered yet. Due to this, this study aims to determine the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis using flow cytometry (FCM) method. Nuclei suspension of selected Cucumis species were extracted using LBO1 lysis buffer by manual chopping technique and stained by propidium iodide priot to FCM analysis. Genome size of C. sativus, C. melo inodorus (Honeydew) and C. melo cantalupensis (Rockmelon) were determined by using Glycine max (Soybean) as an external reference standard (2C = 2.5 pg). This study found that the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis estimated to be 2.83 pg, 3.00 pg and 3.47 pg respectively. The genome size data obtained from this study can be used in future genome studies as well as species characterization.

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流式细胞术测定黄瓜、蜜瓜和岩瓜的基因组大小

葫芦科由具有经济价值和营养价值的物种组成。形态上，Cucumis物种之间只有很少的差异。Cucumis物种基因组大小的种间和种内变异尚未发现。因此，本研究旨在使用流式细胞术（FCM）方法测定马齿苋、甜瓜inodorus和甜瓜cantalupensis的基因组大小。用LBO1裂解缓冲液通过手动切割技术提取所选Cucumis物种的细胞核悬浮液，并用碘化丙啶朊病毒染色进行FCM分析。以大豆（Glycine max）为外参照标准（2C＝2.5pg），测定了C.sativus、C.melo inodorus（Honeydew）和C.melo cantalupensis（Rockmelon）的基因组大小。本研究发现，C.sativus、C.melo inodorus和C.melo cantalupensis的基因组大小估计分别为2.83pg、3.00pg和3.47pg。从这项研究中获得的基因组大小数据可用于未来的基因组研究以及物种表征。

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Science Heritage Journal

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0.00%

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审稿时长

12 weeks