Identification and Functional Characterization of a New Terpene Synthase Gene from Chrysanthemum indicum var. aromaticum

Abstract

in the Chrysanthemum genus. Studies have found that (–)-α-thujone, (+)-β-thujone, borneol and other terpenoids are the main components of C. indicum var. aromaticum (Jian et al. 2014). Terpene synthase (TPS) is a key enzyme that is studied in the biosynthesis of steroids. According to the structure and reaction mechanism, TPS in plants can be mainly divided into two categories. Class I refers to the ion-isation-dependent TPS caused by the removal of the pyrophosphate groups by metal ions such as Mn 2+ and Mg 2+ (Tholl 2015). Class II refers to the proton-ation-dependent TPS that causes the pyrophosphate group to leave by epoxidation or carbon-carbon double bond protonation (Tholl 2015). The TPS family can be divided into seven subfamilies from TPSa-TPSg according to the correlation of its amino acid