Pre-clinical Evaluation and In-silico Docking of Coriandrum sativum on Stress and Cognitive Defi cits in Rodents

Abstract

Ethanolic extract aerial portions of Coriandrum sativum (EECS) were tested for their anxiolytic eff ects on the cold-resistant stress test after seven days of treatment and their cognitive boosting eff ects after eight days of therapy (EECS). Total phenolic content and fl avonoid compound levels were calculated. C. sativum showed anti-stress effi cacy in a cold-resistant stress animal at 200 and 400 mg/kg doses. The plasma glucose, triglyceride, cholesterol levels, as well as the weight of the adrenal glands are all aff ected by the stimulation of the HPA in stressful situations. Pre-treatment with EECS and Geriforte, which contain secondary metabolites such as fl avonoids, glycosides, triterpenoids, and phenolic compounds, signifi cantly reduced stress-induced changes in these biochemical levels in cold stress animals. Screening for acetylcholinesterase inhibition activity in-vitro using Ellman’s approach increased AChE inhibition dose-dependently in the brains of mice. The data proved that the extract’s potential to reduce pain in-vitro is what led to the observed cognitive benefi ts in-vivo. Animals given EECS at 200 and 400 mg/kg had their infl exion ratio improved as a result of the memory defi cit being reversed. The scopolamine-induced amnesia group of mice showed degeneration of vacuolated cells, pyramidal cells, pyknosis, loss of architecture and the creation of lesions in the hippocampus; however, these characteristics were restored by EECS and standard therapy. Eff ective docking scores in mcule software were observed for interactions with receptors PDB: 4K5Y (CRF-1) for adaptogenic and PDB: 1E66 (AChE) for cognition, according to in-silico research. The ethanolic extract of C. sativum has been shown to have positive and scientifi cally-supported eff ects on human health when used as a nutraceutical, with the in-vitro acetylcholinesterase Inhibition assay, anti-stress, and cognitive enhancement activities, and in-silico studies all corroborated by this study.