Comparative Study to Determine Antimicrobial Property of Neem Leaves (Azadirachta indica) Extracts Using Various Solvents Obtained from Two Geographical Area

Journal of Medical Sciences and Health Pub Date : 2023-07-08 DOI:10.46347/jmsh.v9i1.22.317

S. Megha, .. Indradhanush, D. Venkatesha

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Abstract

Background: Plants have been the basis for many medicinal compounds that are used in modern-day medicine, screening for medicinal plants for bioactive compounds is a way of identifying and developing new antimicrobials that are less expensive and with improved safety and efficacy. Objective: The objective of this study was to investigate the in vitro antimicrobial activity of Azadirachta indica (neem) using leaf extracts of Ethanol, Petroleum ether, and Ethyl acetate and testing them on E.coli, Klebsiella, S.aureus, MRSA and C.albicans. Materials & Methods: Minimum inhibitory concentration (MIC) was found using the serial dilution method and antimicrobial susceptibility testing (AST) was done using the disc diffusion method. Results: Ethanolic extract showed better results against Klebsiella and S.aureus, Ethyl acetate extract showed better results against E.coli and MRSA, whereas both ethanolic and petroleum ether extract showed activity against C.albicans. 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay showed that all the extracts have antioxidant property and variations were noted in the antimicrobial activity on comparing samples taken from different locations. Conclusion: Further studies are needed into the bioactive compounds of neem in order to explore their antimicrobial activity. Keywords: Antimicrobial susceptibility, Azadirachta indica, Ethanol, Ethyl acetate, Disc diffusion, MIC, Petroleum ether

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不同溶剂测定印楝叶提取物抗菌性能的比较研究

背景：植物是现代医学中使用的许多药用化合物的基础，筛选药用植物中的生物活性化合物是鉴定和开发成本较低、安全性和有效性提高的新型抗菌药物的一种方法。目的：采用乙醇、石油醚和乙酸乙酯叶提取物对印楝的体外抗菌活性进行研究，并对大肠杆菌、克雷伯菌、金黄色葡萄球菌、，材料与方法：采用连续稀释法测定最低抑菌浓度（MIC），采用纸片扩散法进行药敏试验（AST）。结果：乙醇提取物对克雷伯菌和金黄色葡萄球菌有较好的抗菌效果，乙酸乙酯提取物对大肠杆菌和MRSA有较好的杀菌效果，乙醇和石油醚提取物均对白色念珠菌具有抗菌活性。2，2-二苯基-1-苦基-水合物（DPPH）分析表明，所有提取物都具有抗氧化性能，并且在比较不同地点的样品时，抗菌活性存在差异。结论：印楝的活性成分有待进一步研究，以探索其抗菌活性。关键词：抗菌药物敏感性；印楝；乙醇；乙酸乙酯；圆盘扩散；MIC；石油醚

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Journal of Medical Sciences and Health

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审稿时长

12 weeks