Hypoglycemic effect on adult zebrafish (Danio rerio) of the 3β-6β-16β-trihydroxylup-20(29)-ene triterpene isolated from Combretum leprosum leaves in vivo and in silico approach

IF 2.1 4区医学 Q3 PHARMACOLOGY & PHARMACY

Fundamental & Clinical Pharmacology Pub Date : 2022-03-09 DOI:10.1111/fcp.12776

Marnielle Rodrigues Coutinho, Antonio Wlisses da Silva, Maria Kueirislene Amâncio Ferreira, Emanuela de Lima Rebouças, Francisco Rogênio S. Mendes, Edson Holanda Teixeira, Emanuelle Machado Marinho, Márcia Machado Marinho, Emmanuel Silva Marinho, Alexandre Magno Rodrigues Teixeira, Jane Eire Silva Alencar de Menezes, Hélcio Silva dos Santos

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引用次数: 2

Abstract

Drugs used to manage type 2 diabetes mellitus cause adverse effects. Therefore, the search for new drugs as an alternative for the treatment of diabetes increases. The effect of triterpene 3β-6β-16β-trihydroxylup-20(29)-ene isolated from the leaves of C. leprosum (CLF-1) on sucrose-induced hyperglycemia in adult zebrafish (Danio rerio) was evaluated. Initially, adult zebrafish (n = 6/group) underwent hyperglycemia induction by sucrose at 83.25 mM/L for 7 days by immersion. The hyperglycemic groups were treated with CLF-1 (4, 20, and 40 mg/kg), metformin (200 mg/kg), and acarbose (300 mg/kg) for 4 days. The in silico interaction of CLF-1, metformin, and acarbose with the enzyme maltase-glucoamylase (CtMGAM) was investigated. CLF-1 reduced sucrose-induced hyperglycemia after 4 days of treatment, in addition to having better affinity energy with CtMGAM than metformin and acarbose. Thus, CLF-1 may be a new pharmacological alternative as a hypoglycemic agent for the treatment of diabetes.

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从麻油叶中分离的3β-6β-16β-三羟基羽扇豆-20（29）烯三萜对成年斑马鱼（Danio rerio）的降血糖作用

用于治疗2型糖尿病的药物会引起不良反应。因此，寻找新药作为治疗糖尿病的替代品的呼声越来越高。评估了从C.leprosum（CLF-1）叶片中分离的三萜3β-6β-16β-三羟基lup‐20（29）烯对成年斑马鱼（Danio rerio）蔗糖诱导的高血糖的影响。最初，成年斑马鱼（n = 6/组）在83.25时通过蔗糖诱导高血糖 mM/L浸泡7天。高血糖组用CLF-1（4、20和40 mg/kg）、二甲双胍（200 mg/kg）和阿卡波糖（300 mg/kg）处理4天。研究了CLF‐1、二甲双胍和阿卡波糖与麦芽糖酶-葡糖淀粉酶（CtMGAM）的计算机相互作用。CLF-1在治疗4天后降低了蔗糖诱导的高血糖，此外与二甲双胍和阿卡波糖相比，它与CtMGAM具有更好的亲和力。因此，CLF‐1可能是治疗糖尿病的一种新的药物替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fundamental & Clinical Pharmacology 医学-药学

CiteScore

5.30

自引率

6.90%

发文量

111

审稿时长

6-12 weeks

期刊介绍： Fundamental & Clinical Pharmacology publishes reports describing important and novel developments in fundamental as well as clinical research relevant to drug therapy. Original articles, short communications and reviews are published on all aspects of experimental and clinical pharmacology including: Antimicrobial, Antiviral Agents Autonomic Pharmacology Cardiovascular Pharmacology Cellular Pharmacology Clinical Trials Endocrinopharmacology Gene Therapy Inflammation, Immunopharmacology Lipids, Atherosclerosis Liver and G-I Tract Pharmacology Metabolism, Pharmacokinetics Neuropharmacology Neuropsychopharmacology Oncopharmacology Pediatric Pharmacology Development Pharmacoeconomics Pharmacoepidemiology Pharmacogenetics, Pharmacogenomics Pharmacovigilance Pulmonary Pharmacology Receptors, Signal Transduction Renal Pharmacology Thrombosis and Hemostasis Toxicopharmacology Clinical research, including clinical studies and clinical trials, may cover disciplines such as pharmacokinetics, pharmacodynamics, pharmacovigilance, pharmacoepidemiology, pharmacogenomics and pharmacoeconomics. Basic research articles from fields such as physiology and molecular biology which contribute to an understanding of drug therapy are also welcomed.